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Tags & Cell Markers Subcellular Markers Organelles Caveolae and Clathrin

Anti-Caveolin-2 (phospho Y19)抗体(ab3417)

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Reviews (4) Submit a question References (9)

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Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)

Key features and details

  • Rabbit polyclonal to Caveolin-2 (phospho Y19)
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Caveolin-2 (phospho Y19)抗体
    参阅全部 Caveolin-2 一抗
  • 描述

    兔多克隆抗体to Caveolin-2 (phospho Y19)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide corresponding to Mouse Caveolin-2 aa 1-100 (phospho Y19).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Caveolin-2 protein (ab114515)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3417于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
1/10 - 1/100.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
1/10 - 1/100.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • 组织特异性

    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • 序列相似性

    Belongs to the caveolin family.
  • 翻译后修饰

    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • 细胞定位

    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Target information above from: UniProt accession P51636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 858 Human
    • Omim: 601048 Human
    • SwissProt: P51636 Human
    • Unigene: 212332 Human
    • Unigene: 603096 Human
    • 别名

      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)

      Immunocytochemistry/Immunofluorescence analysis of Phospho-Caveolin-2 pTyr19 (green) showing staining in the cytoplasm and nucleus of HUVEC cells treated with 100µM pervanadate (left) and untreated HUVEC cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3417 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
      IHC image of ab3417 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (ab3417)Image courtesy of Y Pak by Abreview.

      ab3417 at a 1/250 dilution staining Caveolin-2 in rat fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized with Triton X-100 and blocked using 1% BSA. The secondary used was a TRITC conjugated anti-rabbit at a 1/100 dilution.(a) Control cells untreated cells (b) 100nM Insulin for 10 min (c) 10uM U0126 for 2 hr and 100nM Insulin for 10 min (d) 100 nM Wortmannin for 1hr and 100nM Insulin for 10 min

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (9)

    发表研究结果有使用 ab3417?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3417 被引用在 9 文献中.

    • Kwon H  et al. N-myristoylation regulates insulin-induced phosphorylation and ubiquitination of Caveolin-2 for insulin signaling. Biochem Biophys Res Commun 532:535-540 (2020). PubMed: 32896381
    • Liu F  et al. CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway. Onco Targets Ther 11:6209-6216 (2018). PubMed: 30288056
    • Jeong K  et al. Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane. Nucleic Acids Res 43:3114-27 (2015). WB, Immunomicroscopy ; Rat . PubMed: 25753664
    • Kwon H  et al. Fatty acylated caveolin-2 is a substrate of insulin receptor tyrosine kinase for insulin receptor substrate-1-directed signaling activation. Biochim Biophys Acta 1853:1022-34 (2015). PubMed: 25667086
    • Abel B  et al. N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells. FEBS Lett 586:3317-23 (2012). PubMed: 22819829
    • Jeong K  et al. Rab6-mediated retrograde transport regulates inner nuclear membrane targeting of caveolin-2 in response to insulin. Traffic 13:1218-33 (2012). PubMed: 22607032
    • Kwon H  et al. A novel domain of caveolin-2 that controls nuclear targeting: regulation of insulin-specific ERK activation and nuclear translocation by caveolin-2. J Cell Mol Med 15:888-908 (2011). PubMed: 20455999
    • Kwon H  et al. Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin. Biochim Biophys Acta 1793:1325-33 (2009). WB ; Rat . PubMed: 19427337
    • Kwon H  et al. Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis. J Cell Mol Med 13:1549-64 (2009). WB, ICC/IF . PubMed: 19778377

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Caveolin 2 (phospho Y19) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Rat Cell lysate - whole cell (Fibroblasts)
    Total protein in input
    1000 µg
    Specification
    Fibroblasts
    Treatment
    100 nM insulin for 10 min
    Immuno-precipitation step
    Protein A
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Y Pak

    Verified customer

    提交于 Mar 01 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin 2 (phospho Y19) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Fibroblasts)
    Specification
    Fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Y Pak

    Verified customer

    提交于 Mar 01 2010

    Western blot abreview for Anti-Caveolin 2 (phospho Y19) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Fibroblasts)
    Loading amount
    50 µg
    Specification
    Fibroblasts
    Treatment
    100 nM insulin for 10, 30, 60, 120, and 180 min
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Y Pak

    Verified customer

    提交于 Aug 07 2009

    Western blot abreview for Anti-Caveolin 2 (phospho Y19) antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat)
    Loading amount
    1e+006 cells
    Specification
    Jurkat
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 28 2006

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