Anti-Caveolin-1抗体- Caveolae Marker (ab18199)
Key features and details
- Rabbit polyclonal to Caveolin-1 - Caveolae Marker
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caveolin-1抗体- Caveolae Marker
参阅全部 Caveolin-1 一抗 -
描述
兔多克隆抗体to Caveolin-1 - Caveolae Marker -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human, Recombinant fragment
预测可用于: Cow, Dog, Pig
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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| 说明 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
应用说明
Is unsuitable for ICC/IF.
靶标
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功能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
组织特异性
Expressed in muscle and lung, less so in liver, brain and kidney. -
疾病相关
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
序列相似性
Belongs to the caveolin family. -
翻译后修饰
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
细胞定位
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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数据库链接
- Entrez Gene: 281040 Cow
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P79132 Cow
see all -
别名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
图片
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 21-24 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab18199 observed at 21-24 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab18199 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab18199 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Mouse Heart Lysate
Lane 2 : Human Heart Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)Caveolin 1 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse Heart whole tissue lysate, 5µg of Rabbit polyclonal to Caveolin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18199.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 20kDa: Caveolin 1 - Caveolae Marker. -
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml +Recombinant Human Caveolin-1 protein (ab114170) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Exposure time: 1 minuteab18199 recognizes the full length tagged recombinant Caveolin 1 protein (ab114170) which has an expected molecular weight of 46 kDa.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab18199 被引用在 28 文献中.
- Clark A et al. Myogenic tissue nanotransfection improves muscle torque recovery following volumetric muscle loss. NPJ Regen Med 7:63 (2022). PubMed: 36266362
- Biswas S et al. Mural Wnt/β-catenin signaling regulates Lama2 expression to promote neurovascular unit maturation. Development 149:N/A (2022). PubMed: 36098369
- Wang H et al. Inactivation of Hedgehog signal transduction in adult astrocytes results in region-specific blood-brain barrier defects. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34417306
- Reyes L et al. Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo. J Oral Microbiol 12:1742528 (2020). PubMed: 32341760
- Alghamdi AAA et al. NRP2 as an Emerging Angiogenic Player; Promoting Endothelial Cell Adhesion and Migration by Regulating Recycling of a5 Integrin. Front Cell Dev Biol 8:395 (2020). PubMed: 32528960