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Anti-Cathepsin L/MEP抗体(ab58991)

Reviews (4)Q&A (2)References (32)

Key features and details

  • Rabbit polyclonal to Cathepsin L/MEP
  • Suitable for: WB
  • Reacts with: Caenorhabditis elegans
  • Isotype: IgG

概述

  • 产品名称

    Anti-Cathepsin L/MEP抗体
    参阅全部 Cathepsin L/MEP 一抗

  • 描述

    兔多克隆抗体to Cathepsin L/MEP

  • 宿主

    Rabbit

  • 特异性

    Reacts specifically with Cathepsin L/MEP protease 36 kDa wild type protein of C.elegans

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Caenorhabditis elegans

  • 免疫原

    Synthetic peptide corresponding to Caenorhabditis elegans Cathepsin L/MEP. Derived from the mature region of Cathepsin L/MEP (Caenorhabditis elegans)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab58991于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/200 - 1/1000. Predicted molecular weight: 38 kDa.
说明
WB
1/200 - 1/1000. Predicted molecular weight: 38 kDa.

靶标

  • 功能

    Important for the overall degradation of proteins in lysosomes.

  • 序列相似性

    Belongs to the peptidase C1 family.

  • 细胞定位

    Lysosome.
  • Information by UniProt

  • 别名

    • Cathepsin L antibody
    • cathepsin L, 1 b antibody
    • Cathepsin L1 antibody
    • Cathepsin L1 light chain antibody
    • CathepsinL antibody
    • CATL antibody
    • CATL1_HUMAN antibody
    • cb15 antibody
    • CTSL antibody
    • CTSL1 antibody
    • ctsl1b antibody
    • FLJ31037 antibody
    • hgg1 antibody
    • Major excreted protein antibody
    • MEP antibody
    • MGC123162 antibody
    • wu:fb30g09 antibody
    see all

文献 (32)

发表研究结果有使用 ab58991？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab58991 被引用在 32 文献中.

  • Ghosh A  et al. PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase. Life Sci Alliance 6:N/A (2023). PubMed: 37316298
  • Komlós M  et al. The Small-Molecule Enhancers of Autophagy AUTEN-67 and -99 Delay Ageing in Drosophila Striated Muscle Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37175806
  • Chaudhry N  et al. Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. Autophagy 18:2443-2458 (2022). PubMed: 35266854
  • Xu L  et al. ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag. Cell Rep 41:111583 (2022). PubMed: 36323251
  • Hodgson JJ  et al. Identification of Cellular Genes Involved in Baculovirus GP64 Trafficking to the Plasma Membrane. J Virol 96:e0021522 (2022). PubMed: 35608346
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

IHC - Wholemount abreview for Anti-Cathepsin L antibody

 Good
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Zebrafish Embryo (Hatching Gland)
Specification
Hatching Gland
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 11 2015

Western blot abreview for Anti-Cathepsin L antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (kidney)
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 11 2014

Western blot abreview for Anti-Cathepsin L antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 09 2009

Western blot abreview for Anti-Cathepsin L antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
10 µg
Specification
HCT116
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 08 2009

Question

Tanks so much for your reply, and the huge antibody list :-)
Would you please send us more information about the EM
application of the antibody listed below? Publications or EM
images with the protocol will be very helpful.
58991 Rabbit polyclonal to Cathepsin L Hu, Ms, Ce
EM, ICC/IF, WB

Read More
Answer

Thank you for contacting us.

The supplying lab let me know that the antibody was used in EM based on the following information:



Immuno electron microscopy (IEM)
C. elegans gravid hermaphrodites were permeabilised by cutting with a razor blade in fixative (0.5% glutaraldehyde/2% paraformaldehyde, pH 7.4) and incubated in fixative for 1 hour on ice. Following several washes in PBS, worms were embedded in 1.5% agarose, dehydrated through a graded series of alcohol and infiltrated with LR White resin at –20°C. Small clusters were UV-cured in gelatin capsules at room temperature for four days. Thin sections (80 mm) were collected on formvar-coated nickel mesh grids and incubated with the anti-CPL-1 antibody. Gold-conjugated secondary antibody (10 nm or 15 nm gold) (other company) was used to detect antibody binding. Grids were contrast stained by standard procedure in uranyl acetate and lead citrate for EM viewing. Images were captured using Easy Vision software.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Tanks so much for your reply, and the huge antibody list :-)
Would you please send us more information about the EM
application of the antibody listed below? Publications or EM
images with the protocol will be very helpful.
58991 Rabbit polyclonal to Cathepsin L Hu, Ms, Ce
EM, ICC/IF, WB

Read More
Answer

Thank you for your email.

I am checking this information with the lab and let you know what I find out.

Currently, we are not aware of publications that have used the antibody in EM so far.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

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