Anti-Cathepsin B抗体(ab30443)
Key features and details
- Rabbit polyclonal to Cathepsin B
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cathepsin B抗体
参阅全部 Cathepsin B 一抗 -
描述
兔多克隆抗体to Cathepsin B -
宿主
Rabbit -
经测试应用
适用于: ELISA, IP, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified): Cathepsin B from Human liver.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration. PubMed: 24833013
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. PubMed: 24833013 |
|
ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
序列相似性
Belongs to the peptidase C1 family. -
细胞定位
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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别名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab30443 被引用在 5 文献中.
- Chien WW et al. Unexpected cross-reactivity of anti-cathepsin B antibodies leads to uncertainties regarding the mechanism of action of anti-CD20 monoclonal antibody GA101. Leuk Res 55:41-48 (2017). PubMed: 28122282
- Han X et al. AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD(+) elevation. Aging Cell 15:416-27 (2016). PubMed: 26890602
- Lohoefer F et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . PubMed: 24833013
- Li C et al. Expression and clinical significance of cathepsin B and stefin A in laryngeal cancer. Oncol Rep 26:869-75 (2011). PubMed: 21667036
- Krohne TU et al. Lipid peroxidation products reduce lysosomal protease activities in human retinal pigment epithelial cells via two different mechanisms of action. Exp Eye Res 90:261-6 (2010). PubMed: 19895809