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使用敲除细胞株进行验证

Anti-Catalase抗体[12C2DB9] - Peroxisome Marker (ab110292)

Reviews (3)Q&A (1)References (3)
Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

Key features and details

  • Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
  • Suitable for: ICC, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Catalase抗体[12C2DB9] - Peroxisome Marker
    参阅全部 Catalase 一抗

  • 描述

    小鼠单克隆抗体[12C2DB9] to Catalase - Peroxisome Marker

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, IP, Flow Cytmore details

  • 种属反应性

    与反应: Rat, Cow, Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • 阳性对照

    • HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver Flow cyt: HAP1-WT cells.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110292于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

  • 疾病相关

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

  • 序列相似性

    Belongs to the catalase family.

  • 翻译后修饰

    The N-terminus is blocked.

  • 细胞定位

    Peroxisome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

图片

  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-CAT knockout cells (red line) stained with ab110292. The cells were fixed with 4% formaldehyde (10 min)  and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab110292, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

    A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CAT  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
  • Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunoprecipitation of Catalase using ab110292 from:
    Lane 1: HepG2 cells
    Lane 2: Cow heart sample
    Lane 3: Rat liver sample

    Predicted band size is 60 kDa.
  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

文献 (3)

发表研究结果有使用 ab110292？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110292 被引用在 3 文献中.

  • Covill-Cooke C  et al. Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2. EMBO Rep 21:e49865 (2020). PubMed: 31894645
  • Chang CL  et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol 218:2583-2599 (2019). PubMed: 31227594
  • Gut G  et al. Multiplexed protein maps link subcellular organization to cellular states. Science 361:N/A (2018). PubMed: 30072512

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (COS-7)
Permeabilization
Yes - Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 06 2022

Immunocytochemistry abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
African green monkey Cultured Cells (Vero cells)
Permeabilization
Yes - Triton X-100 0.2%
Specification
Vero cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 07 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HFF cells)
Permeabilization
Yes - Triton X-100
Specification
HFF cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 23 2021

Question

I was interested in buying some Catalase antibody and was wondering what kind of Kd values they have towards bovine catalase?

Read More
Answer

Thank you very much for contacting us.

Unfortunately, the dissociation constant KD is not routinely determined and we therefore cannot provide this information on our antibodies against bovine catalase.

Having said this, I am happy to let you know that we have at least six anti-catalase antibodies that have been tested with and are guaranteed to work with bovine samples. These include for example the rabbit polyclonal ab1877 or the mouse monoclonal ab110292:

Click here (or use the following: https://www.abcam.com/Catalase-antibody-ab1877.html).

Click here (or use the following: https://www.abcam.com/Catalase-antibody-12C2DB9-Peroxisome-Marker-ab110292.html).

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More

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