Anti-CAST抗体(ab76933)
Key features and details
- Rabbit polyclonal to CAST
- Suitable for: WB, IP, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-CAST抗体
参阅全部 CAST 一抗 -
描述
兔多克隆抗体to CAST -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide corresponding to Human CAST aa 400-500. NP_036231.1
Database link: O15446-1 -
阳性对照
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate; IHC: human breast carcinoma; ICC: HeLa cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab76933 was affinity purified using an epitope specific to CAST immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 55 kDa.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| ICC |
1/50 - 1/2000.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells |
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 55 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
ICC
1/50 - 1/2000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. -
序列相似性
Belongs to the eukaryotic RPA34 RNA polymerase subunit family. -
翻译后修饰
Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. - Information by UniProt
-
数据库链接
- Entrez Gene: 10849 Human
- Omim: 107325 Human
- SwissProt: O15446 Human
- Unigene: 710495 Human
-
别名
- A34.5 antibody
- Anti sense to ERCC 1 protein antibody
- Antisense to ERCC-1 protein antibody
see all
图片
-
Immunocytochemistry - Anti-CAST antibody (ab76933)
Immunocytochemical analysis of formaldehyde-fixed asynchronous HeLa cells aataining CAST with ab76933 at 1/100 dilution and detected by Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed antibody at 1/100 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (ab76933)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAST with ab76933 at 1/200 (1µg/ml). Detection: DAB.
-
Western blot - Anti-CAST antibody (ab76933)All lanes : Anti-CAST antibody (ab76933) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-CAST antibody (ab76933)ab76933 at 1µg/ml staining CAST in HeLa whole cell lysate immunoprecipitated using ab76933 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab76933 尚未被引用在任何文献中。