AB223360

Anti-CaSR抗体

Name: CaSR抗体 (ab223360)| Abcam中文官网
Brand: Abcam Limited
SKU: AB223360
Price: 4890 CNY
Availability: InStock

Anti-CaSR antibody

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal CASR antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CASR aa 1-100.

查看别名

GPRC2A, PCAR1, CASR, Extracellular calcium-sensing receptor, CaR, CaSR, hCasR, Parathyroid cell calcium-sensing receptor 1, PCaR1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (AB223360)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (AB223360)

4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.

(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.

Supplier Data

Western blot - Anti-CaSR antibody (AB223360)

Blocking buffer : 5% skim milk in TBST.

Primary incubation : 16 hours at 4°C.

Color Development : TMB.

All lanes:

Western blot - Anti-CaSR antibody (ab223360) at 1/1000 dilution

All lanes:

Rat kidney lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/200 dilution

Predicted band size: 121 kDa

Observed band size: 130 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human CASR aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P41180

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The calcium-sensing receptor often referred to as CaSR is a critical G-protein coupled receptor involved in calcium ion homeostasis. Also known as CASR this receptor detects changes in extracellular calcium concentration and modulates signaling pathways accordingly. The CaSR protein has a molecular weight of approximately 120 kDa. It is mainly expressed in the parathyroid gland kidney and several other tissues playing a significant role in regulating serum calcium levels.

Biological function summary

The calcium-sensing receptor modulates parathyroid hormone secretion and renal calcium excretion in response to calcium fluctuations. Although not typically part of a larger protein complex it interacts with several other proteins to perform its functions efficiently. CaSR ensures the stability of calcium concentration which is essential for bone health muscle contraction and nerve function.

Pathways

Several other proteins work alongside the calcium-sensing receptor in the calcium signaling and parathyroid hormone secretion pathways. In particular CaSR influences the MAPK pathway which impacts cell growth and differentiation. Phosphate transporters and calbindins are related to CaSR within these pathways indicating its broad scope in calcium metabolism.

The calcium-sensing receptor is closely associated with conditions like hypercalcemia and autosomal dominant hypocalcemia. CaSR mutations can result in dysfunctional calcium regulation impacting parathyroid hormone levels. The relationship between CaSR and parathyroid hormone-related peptide highlights its significance in maintaining calcium balance and preventing skeletal and neurological complications.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed : 17555508, PubMed : 19789209, PubMed : 21566075, PubMed : 22114145, PubMed : 22789683, PubMed : 23966241, PubMed : 25104082, PubMed : 25292184, PubMed : 25766501, PubMed : 26386835, PubMed : 7759551, PubMed : 8636323, PubMed : 8702647, PubMed : 8878438). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (By similarity). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism : aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed : 27386547, PubMed : 27434672).

See full target information CASR

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 35:102311 PubMed41050091

2025

Targeting chaperone-mediated autophagy to regulate osteoclast activity as a therapeutic strategy for osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhui Zhang,Quanfeng Li,Xiaoshuai Peng,Pengfei Ji,Yibin Zhang,Jiahao Jin,Zihao Yuan,Jianan Jiang,Guangqi Tian,Mingxi Cai,Pei Feng,Yanfeng Wu,Wenjie Liu,Peng Wang

Translational research : the journal of laboratory and clinical medicine 265:17-25 PubMed37990828

2023

Repurposing calcium-sensing receptor activator drug cinacalcet for ADPKD treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Pattareeya Yottasan,Tifany Chu,Parth D Chhetri,Onur Cil

International journal of molecular sciences 23: PubMed36362064

2022

Attenuation of Myocardial Dysfunction in Hypertensive Cardiomyopathy Using Non-R-Wave-Synchronized Cardiac Shock Wave Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Li,Zhe Zhen,Si-Jia Sun,Yu Jiang,Wei-Hao Liang,Markus Belau,Rafael Storz,Song-Yan Liao,Hung-Fat Tse

Experimental and therapeutic medicine 20:818-829 PubMed32742325

2020

Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system.

Applications

Unspecified application

Species

Unspecified reactive species

Yongli Zhao,Na Tang,Dongmei Xi,Zhen Huang,Tian Zhang,Yongmin Liu,Lamei Wang,Yan Tang,Hua Zhong,Fang He

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaSR抗体

Anti-CaSR antibody

Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (AB223360)

Western blot - Anti-CaSR antibody (AB223360)