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AB79202

Anti-Caspase-9 (phospho Y153)抗体

Anti-Caspase-9 (phospho Y153) antibody

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(2 Publications)

Rabbit Polyclonal Caspase-9 phospho Y153 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 phospho Y153.

查看别名

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

Left panel : immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel : same, after pre-incubation with the immunizing phosphopeptide.

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

Left panel : immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel : same, after pre-incubation with the immunizing phosphopeptide.

Western blot - Anti-Caspase-9 (phospho Y153) antibody (AB79202)
  • WB

Unknown

Western blot - Anti-Caspase-9 (phospho Y153) antibody (AB79202)

All lanes:

Western blot - Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution

Lane 1:

COLO205 cell extracts at 30 µg

Lane 2:

COLO205 cell extracts at 30 µg with immunizing peptide

Predicted band size: 46 kDa

Observed band size: 40 kDa,46 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human CASP9 phospho Y153. The exact immunogen used to generate this antibody is proprietary information.

P55211

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.
See full target information CASP9 phospho Y153
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 11: PubMed36552682

2022

Sidestream Smoke Extracts from Harm-Reduction and Conventional Camel Cigarettes Inhibit Osteogenic Differentiation via Oxidative Stress and Differential Activation of intrinsic Apoptotic Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole R L Sparks,Lauren M Walker,Steven R Sera,Joseph V Madrid,Michael Hanna,Edward C Dominguez,Nicole I Zur Nieden

Journal of cellular biochemistry 120:836-847 PubMed30125989

2018

Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.

Applications

Unspecified application

Species

Unspecified reactive species

Weijun Gong,Shuyan Qie,Peiling Huang,Jianing Xi
View all publications
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