AB210611

Anti-Caspase-9 p35抗体

Name: Caspase-9 p35抗体 (ab210611)| Abcam中文官网
Brand: Abcam Limited
SKU: AB210611
Price: 4797 CNY
Availability: InStock

Anti-Caspase-9 p35 antibody

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(3 Publications)

Rabbit Polyclonal Caspase-9 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

查看别名

MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 p35 antibody (AB210611)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 p35 antibody (AB210611)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Caspase-9 p35 with ab210611 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-Caspase-9 p35 antibody (AB210611)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-9 p35 antibody (AB210611)

Intracellular flow cytometric analysis of RSC96cells labeling Caspase-9 p35with ab210611 at 1/100 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Supplier Data

Western blot - Anti-Caspase-9 p35 antibody (AB210611)

All lanes:

Western blot - Anti-Caspase-9 p35 antibody (ab210611) at 1/300 dilution

All lanes:

Raji lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

Predicted band size: 46 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

IHC-P, WB, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human CASP9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P55211

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed : 36758105, PubMed : 36758106).. Isoform 2. Lacks activity is an dominant-negative inhibitor of caspase-9.

See full target information CASP9

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303946 PubMed37897313

2023

Impaired Efferocytosis Enables Apoptotic Osteoblasts to Escape Osteoimmune Surveillance During Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyao Xu,Hanyu Xie,Xin Shen,Jiadong Huang,Hengguo Zhang,Yu Fu,Ping Zhang,Songsong Guo,Dongmiao Wang,Sheng Li,Kai Zheng,Wen Sun,Laikui Liu,Jie Cheng,Hongbing Jiang

Neoplasma 68:362-374 PubMed33118833

2020

LncRNA MEG3 promotes endoplasmic reticulum stress and suppresses proliferation and invasion of colorectal carcinoma cells through the MEG3/miR-103a-3p/PDHB ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqin Wang,Qiuwen Ye,Shujuan Ning,Zhuangqing Yang,Yunlan Chen,Lijuan Zhang,Youguang Huang,Fujia Xie,Xi Cheng,Junlin Chi,Yi Lei,Rong Guo,Jiangqiong Han

EBioMedicine 46:54-65 PubMed31324603

2019

Systematic cancer-testis gene expression analysis identified CDCA5 as a potential therapeutic target in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Chengxiang Zhu,Yue Yu,Weibing Wu,Jing Cao,Zhihua Li,Juncheng Dai,Cheng Wang,Yu Tang,Quan Zhu,Jun Wang,Wei Wen,Lei Xue,Fuxi Zhen,Jinyuan Liu,Chenjun Huang,Fei Zhao,Yue Zhou,Zhicheng He,Xianglong Pan,Haixing Wei,Yining Zhu,Yaozhou He,Jun Que,Jinghua Luo,Liang Chen,Wei Wang

View all publications

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Anti-Caspase-9 p35抗体

Anti-Caspase-9 p35 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 p35 antibody (AB210611)

Flow Cytometry (Intracellular) - Anti-Caspase-9 p35 antibody (AB210611)

Western blot - Anti-Caspase-9 p35 antibody (AB210611)