重组Anti-Caspase-9抗体[EPR18868] (ab184786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18868] to Caspase-9
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-Caspase-9抗体[EPR18868]
参阅全部 Caspase-9 一抗 -
描述
兔单克隆抗体[EPR18868] to Caspase-9 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain and heart lysates. IP: NIH/3T3 and C6 treated with 1µM staurosporine for 4h whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18868 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184786于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/30.
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WB | (1) |
1/1000. Detects a band of approximately 50, 39, 37 kDa (predicted molecular weight: 50, 39, 37 kDa).
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说明 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 50, 39, 37 kDa (predicted molecular weight: 50, 39, 37 kDa). |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
组织特异性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
发展阶段
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻译后修饰
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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数据库链接
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- SwissProt: Q8C3Q9 Mouse
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别名
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
图片
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 0.5 hour
Lane 3 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour
Lane 4 : C6 whole cell lysate treated with 1 µm staurosporine for 4 hours
Lane 5 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour, then treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The p39 and p37 subunits were inhibited when the caspase activity was blocked by the caspase inhibitor, Z-VAD-FMK.
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Caspase 9 was immunoprecipitated from 1mg of NIH/3T3 whole cell lysate (Mouse embyro fibroblast cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h,10ug (Input).
Lane 2: ab184786 IP in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could not be observed even with 3 minutes exposure time.
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Caspase 9 was immunoprecipitated from 1mg of C6 whole cell lysate (Rat glial tumor cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate treated with 1μM staurosporine for 4h, 10ug (Input).
Lane 2: ab184786 IP in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could be observed with longer exposure time.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (44)
ab184786 被引用在 44 文献中.
- Tang Z et al. Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats. Aging (Albany NY) 15:12264-12274 (2023). PubMed: 37934569
- Weng H et al. Anti-oxidative-initiated cognitive impairment amelioration in Alzheimer's disease model rats through preventive transcutaneous electrical acupoint stimulation. Integr Med Res 12:100946 (2023). PubMed: 37187681
- Tao W et al. Optimal treatment for post-MI heart failure in rats: dapagliflozin first, adding sacubitril-valsartan 2 weeks later. Front Cardiovasc Med 10:1181473 (2023). PubMed: 37383701
- Zhang Q et al. Sevoflurane exposure causes neuronal apoptosis and cognitive dysfunction by inducing ER stress via activation of the inositol 1, 4, 5-trisphosphate receptor. Front Aging Neurosci 14:990679 (2022). PubMed: 36337694
- Yang L et al. Taurine attenuates ER stress-associated apoptosis and catabolism in nucleus pulposus cells. Mol Med Rep 25:N/A (2022). PubMed: 35315493