重组Anti-Caspase-9抗体[E84] (ab32068)
![Western blot - Anti-Caspase-9 antibody [E84] (ab32068)](https://www.abcam.cn/ps/products/32/ab32068/Images/ab32068-277443-anti-caspase-9-antibody-e84-western-blot.jpg "Western blot - Anti-Caspase-9 antibody [E84] (ab32068)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E84] to Caspase-9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Caspase-9抗体[E84]
参阅全部 Caspase-9 一抗 -
描述
兔单克隆抗体[E84] to Caspase-9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Caspase-9 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- Jurkat cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E84 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
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| IHC-P |
1/25. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
|
IHC-P
1/25. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
组织特异性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
发展阶段
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻译后修饰
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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数据库链接
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
- Unigene: 329502 Human
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别名
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
图片
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Western blot - Anti-Caspase-9 antibody [E84] (ab32068)
Lanes:
Lane 1: Wild-type HAP1 whole cell lysate (40 µg).
Lane 2: CASP9 knockout HAP1 whole cell lysate (40µg).
Lane 3: HeLa whole cell lysate (40 µg).Lanes 1 - 3 Merged signal (red and green). Green - ab32068 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32068 was shown to specifically recognize CASP9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CASP9 knockout samples were examined. Wild-type and CASP9 knockout samples were subjected to SDS-PAGE. Ab32068 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-9 antibody [E84] (ab32068)](https://www.abcam.cn/ps/products/32/ab32068/Images/ab32068-279861-anti-caspase-9-antibody-e84-western-blot.png)
Western blot - Anti-Caspase-9 antibody [E84] (ab32068)All lanes : Anti-Caspase-9 antibody [E84] (ab32068) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with staurosporine 1uM for 4 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Additional bands at: 35 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)](https://www.abcam.cn/ps/products/32/ab32068/Images/ab32068-43789-anti-caspase-9-antibody-e84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] (ab32068)Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Caspase-9 antibody [E84] (ab32068)](https://www.abcam.cn/ps/products/32/ab32068/Images/ab32068-43790-anti-caspase-9-antibody-e84-western-blot.jpg)
Western blot - Anti-Caspase-9 antibody [E84] (ab32068)Anti-Caspase-9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Predicted band size: 46 kDa
Observed band size: 46 kDa -
![Anti-Caspase-9 antibody [E84] (ab32068)](https://www.abcam.cn/ps/products/32/ab32068/Images/ab32068-5-benefits-of-recombinant-antibodies.png)
Anti-Caspase-9 antibody [E84] (ab32068)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab32068 被引用在 15 文献中.
- An W et al. Alpinia katsumadai Hayata induces growth inhibition and autophagy‑related apoptosis by regulating the AMPK and Akt/mTOR/p70S6K signaling pathways in cancer cells. Oncol Rep 48:N/A (2022). PubMed: 35730618
- Yang Q et al. Lidocaine alleviates cytotoxicity-resistance in lung cancer A549/DDP cells via down-regulation of miR-21. Mol Cell Biochem 456:63-72 (2019). PubMed: 30644017
- Zhao J et al. Salidroside mitigates hydrogen peroxide-induced injury by enhancement of microRNA-27a in human trabecular meshwork cells. Artif Cells Nanomed Biotechnol 47:1758-1765 (2019). PubMed: 31062616
- Meng FC & Lin JK Liquiritigenin Inhibits Colorectal Cancer Proliferation, Invasion, and Epithelial-to-Mesenchymal Transition by Decreasing Expression of Runt-Related Transcription Factor 2. Oncol Res 27:139-146 (2019). PubMed: 29471888
- Sun T et al. Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis. Cell Physiol Biochem 45:832-843 (2018). PubMed: 29414810