为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cell Biology Apoptosis Intracellular Caspases etc Caspases

Anti-Caspase-9抗体(ab2013)

  • Datasheet
Reviews (2)Q&A (5)References (33)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)

Key features and details

  • Rabbit polyclonal to Caspase-9
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant human Caspase-9 protein (Tagged) (ab198061)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一抗
Product image
Anti-beta Actin antibody [AC-15] (ab6276)

查看更多相关产品

概述

  • 产品名称

    Anti-Caspase-9抗体
    参阅全部 Caspase-9 一抗
  • 描述

    兔多克隆抗体to Caspase-9
  • 宿主

    Rabbit
  • 特异性

    No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
    Sequence:

    EDIQRAGSGSRRDQAR


    Database link: P55211
    (Peptide available as ab7885)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Caspase-9 Antibody is affinity chromatography purified via peptide column.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Cytochrome C
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Cytochrome C
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

相关产品

  • Assay kits

    • Caspase 9 Assay Kit (Colorimetric) (ab65608)
    • Caspase 9 (active) FITC Staining Kit (ab65615)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Caspase-9 protein (Tagged) (ab198061)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2013于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
说明
WB
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 组织特异性

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 发展阶段

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻译后修饰

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Target information above from: UniProt accession P55211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 842 Human
    • Entrez Gene: 12371 Mouse
    • Entrez Gene: 58918 Rat
    • Omim: 602234 Human
    • SwissProt: P55211 Human
    • SwissProt: Q8C3Q9 Mouse
    • Unigene: 329502 Human
    • 别名

      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    图片

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      All lanes : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Lane 1 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
      Lane 2 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml
      Lane 3 : Anti-Caspase-9 antibody (ab2013) at 4 µg/ml

      All lanes : Human heart lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Lane 1 : Anti-Caspase-9 antibody (ab2013) at 0.5 µg/ml
      Lane 2 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
      Lane 3 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml

      All lanes : Mouse spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      All lanes : Anti-Caspase-9 antibody (ab2013) at 0.5 µg

      All lanes : Rat spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

      Predicted band size: 46 kDa
      Observed band size: 46 kDa

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (33)

    发表研究结果有使用 ab2013?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2013 被引用在 33 文献中.

    • Meng L  et al. Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells. Mol Med Rep 23:N/A (2021). PubMed: 33786628
    • Wang Y  et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
    • Liu H  et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). PubMed: 33356694
    • Lv H  et al. Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429. Cell Cycle 19:207-217 (2020). PubMed: 31876239
    • Ren DL  et al. Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. Oncol Lett 19:255-260 (2020). PubMed: 31897137
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Caspase-9 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - other (Lion, muscle tissue)
    Gel Running Conditions
    Reduced Denaturing (8-16%)
    Loading amount
    150 µg
    Specification
    Lion, muscle tissue
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mayra González

    Verified customer

    提交于 Jul 14 2016

    Western blot abreview for Anti-Caspase 9 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (stomach cancer)
    Loading amount
    25 µg
    Specification
    stomach cancer
    Treatment
    10uM WIN 55,212-2 for 24hrs
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 27 2013

    Question

    DESCRIPTION OF THE PROBLEM Does the casp9 antibody ab2013 detect your casp9 protein ab52203? If not can you recommend an antibody that will detect it so I can order the protein and ab together next week? I would like to perform a binding / inhibition experiment using these products if they are compatible.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2011

    Answer

    Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2011

    Question

    Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2006

    Answer

    Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

    Read More

    Abcam Scientific Support

    回复于 Mar 20 2006

    Question

    Antibody MUST recognise the cleaved (and precursor) of Caspase 8 and 9 in WB

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2006

    Answer

    Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are as follows: ab28142 detects ~55 and 57 kDa proteins, corresponding to the apparent molecular masses of the proform of caspase-8 alpha and beta on SDS-PAGE immunoblots. In apoptotic cells, this antibody also detects the processed forms (42/44 kDa, 31, 26, 18 and 14kDa). ab2013 detects both the activated (35 or 37kDa cleaved fragment depending on activator) and non-activated caspase-9 (45kDa). I would recommend that you have a look at the antibody's respective datasheets by clicking on the links below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Feb 24 2006

    Question

    has anybody tried caspase 9 for immunohistochemistry? is there any information on this?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2002

    Answer

    No, to our knowledge, this anitbody has only been tested in WB.

    Read More

    Abcam Scientific Support

    回复于 Mar 13 2002

    Question

    DOES THIS AB RECOGNIZE MOUSE CASPASE 9? CAN THIS AB DETECT ONLY THE 46 KDA PROTEIN? DOES IT CROSS REACT WITH THE ACTIVE CASPASE 9 (36 KDA)?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2000

    Answer

    This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not tested the activated form. Our Western Blot tests show a strong, single band at 46kDa. It is human, mouse, and rat reactive.

    Read More

    Abcam Scientific Support

    回复于 Dec 14 2000

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有