Anti-Caspase-8抗体(ab25901)
Key features and details
- Rabbit polyclonal to Caspase-8
- Suitable for: ICC, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caspase-8抗体
参阅全部 Caspase-8 一抗 -
描述
兔多克隆抗体to Caspase-8 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Database link: Q14790 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Caspase-8 Antibody is affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25901于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
Human samples only |
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| ICC/IF |
Use a concentration of 20 µg/ml.
Human samples only |
|
| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Human samples only |
|
ICC/IF
Use a concentration of 20 µg/ml. Human samples only |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
靶标
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功能
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
组织特异性
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. -
疾病相关
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. -
序列相似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
结构域
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. -
翻译后修饰
Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 841 Human
- Entrez Gene: 12370 Mouse
- Entrez Gene: 64044 Rat
- Omim: 601763 Human
- SwissProt: Q14790 Human
- SwissProt: O89110 Mouse
- SwissProt: Q9JHX4 Rat
- Unigene: 599762 Human
see all -
别名
- ALPS2B antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
- Apoptosis related cysteine peptidase antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)Immunocytochemistry/Immunofluorescence of Jurkat cells labeling Caspase-8 with Anti-Caspase-8 antibody (ab25901) at 20μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (151)
ab25901 被引用在 151 文献中.
- Zhou Y et al. Receptor-interacting protein 1 inhibition prevents mechanical stress-induced temporomandibular joint osteoarthritis by regulating apoptosis and later-stage necroptosis of chondrocytes. Arch Oral Biol 147:105612 (2023). PubMed: 36603515
- Lin J et al. Fas/FasL and Complement Activation are Associated with Chronic Active Epstein-Barr Virus Hepatitis. J Clin Transl Hepatol 11:540-549 (2023). PubMed: 36969885
- Zhao D et al. Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury. Front Pharmacol 14:1171293 (2023). PubMed: 37274104
- Zhang D et al. Inhibition of GSK3β activity alleviates acute liver failure via suppressing multiple programmed cell death. J Inflamm (Lond) 20:24 (2023). PubMed: 37443080
- Budijanto S et al. Dietary supplementation of black rice bran to colon carcinogen-induced mice: Examining the development of colorectal cancer by improving environmental colon conditions. Heliyon 9:e18528 (2023). PubMed: 37576300