AB185645

重组Anti-Caspase-6/CASP-6抗体[EPR18043]

Anti-Caspase-6/CASP-6 antibody [EPR18043]

RabMAb
Recombinant
了解详情

(1 Review)

(17 Publications)

Rabbit Recombinant Monoclonal Caspase-6/CASP-6 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 17 publications.

查看别名

MCH2, CASP6, Caspase-6, CASP-6, CSP-6, Apoptotic protease Mch-2

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-6/CASP-6 with ab185645 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing both nuclear and cytoplasmic staining on Jurkat cells. The staining remained similar after treatment with staurosporine (1uM, 4 hours) as the antibody interacts with the subunit p11.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab185645 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic and nuclear staining on epithelial cells of human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on neurons of rat brain is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on neurons of mouse brain is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Caspase-6/CASP-6 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) treated with 1uM staurosporine for 4 hours whole cell lysate with ab185645 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185645 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate10 μg (Input). Lane 2 : ab185645 IP in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab185645 in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate.

The cleaved Caspase-6/CASP-6 appears slightly larger than 11kDa. This fragment contains subunit p11 plus the internal propeptide.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

Predicted band size: 33 kDa

Observed band size: 11 kDa,33 kDa

false

Supplier Data

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

Lane 1:

Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 11 kDa,33 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 11 kDa,33 kDa

false

Exposure time: 1min

不同偶联物与剂型 (1)

Carrier free

Anti-Caspase-6/CASP-6 antibody [EPR18043] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18043

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed : 19133298, PubMed : 22858542, PubMed : 27032039, PubMed : 28864531, PubMed : 30420425, PubMed : 32298652, PubMed : 8663580). Acts as a non-canonical executioner caspase during apoptosis : localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed : 11953316, PubMed : 17401638, PubMed : 8663580, PubMed : 9463409). Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution (PubMed : 11953316). Acts as a regulator of liver damage by promoting hepatocyte apoptosis : in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis (PubMed : 32029622). Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity). Involved in intrinsic apoptosis by mediating cleavage of RIPK1 (PubMed : 22858542). Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed : 10559921, PubMed : 14657026). Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity). In addition to apoptosis, involved in different forms of programmed cell death (PubMed : 32298652). Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity (PubMed : 32298652). PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis (PubMed : 32298652). Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed : 32298652). Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity). Regulates B-cell programs both during early development and after antigen stimulation (By similarity).. (Microbial infection) Proteolytically cleaves the N protein of coronaviruses such as MERS-CoV and SARS-CoV (PubMed : 18155731, PubMed : 35922005). The cleavage of MERS-CoV N-protein leads to two fragments and modulates coronavirus replication by regulating IFN signaling. The two fragments produced by the cleavage interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes (PubMed : 35922005). The same mechanism seems to be used by other coronaviruses such as SARS-CoV and SARS-CoV-2 to enhance their replication (PubMed : 35922005).

See full target information CASP6

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449

2023

Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Ruijiang Lin,Minjie Ma,Biao Han,Ya Zheng,Yuping Wang,Yongning Zhou

Cell death discovery 9:106 PubMed36977670

2023

Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Sheng,Yiqi Weng,Yingli Cao,Chen Zhang,Yuanbang Lin,Wenli Yu

Nature 609:785-792 PubMed35922005

2022

Coronaviruses exploit a host cysteine-aspartic protease for replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hin Chu,Yuxin Hou,Dong Yang,Lei Wen,Huiping Shuai,Chaemin Yoon,Jialu Shi,Yue Chai,Terrence Tsz-Tai Yuen,Bingjie Hu,Cun Li,Xiaoyu Zhao,Yixin Wang,Xiner Huang,Kin Shing Lee,Cuiting Luo,Jian-Piao Cai,Vincent Kwok-Man Poon,Chris Chung-Sing Chan,Anna Jinxia Zhang,Shuofeng Yuan,Ko-Yung Sit,Dominic Chi-Chung Foo,Wing-Kuk Au,Kenneth Kak-Yuen Wong,Jie Zhou,Kin-Hang Kok,Dong-Yan Jin,Jasper Fuk-Woo Chan,Kwok-Yung Yuen

Journal of neurotrauma 39:577-589 PubMed35152732

2022

RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but Not Hippocampal Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Farooq,Kara Snyder,Keri Janesko-Feldman,Kiersten Gorse,Vincent A Vagni,Patrick M Kochanek,Travis C Jackson

Cell death discovery 7:290 PubMed34642327

2021

Downregulation of phosphoglycerate mutase 5 improves microglial inflammasome activation after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Chen,Kai Gong,Limin Guo,Bingchang Zhang,Sifang Chen,Zhangyu Li,Xu Quanhua,Wei Liu,Zhanxiang Wang

Cell death and differentiation 29:709-721 PubMed34621049

2021

Negative feedback of SNRK to circ-SNRK regulates cardiac function post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yan Wang,Xiao-Xiao Liu,Yun-Fei Deng

mBio 12:e0100521 PubMed34125604

2021

Caspase-Dependent Cleavage of DDX21 Suppresses Host Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wu,Yang Qu,Shengqing Yu,Sa Wang,Yuncong Yin,Qinfang Liu,Chunchun Meng,Ying Liao,Zaib Ur Rehman,Lei Tan,Cuiping Song,Xusheng Qiu,Weiwei Liu,Chan Ding,Yingjie Sun

Experimental gerontology 147:111267 PubMed33548486

2021

Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Sira Karvinen,Hanna-Kaarina Juppi,Gengyun Le,Christine A Cabelka,Tara L Mader,Dawn A Lowe,Eija K Laakkonen

Frontiers in cell and developmental biology 8:624985 PubMed33520998

2021

Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Qiang Liu,Yanhong Chen,Huizhen Ye,Han Wang,Shenming Zeng

Cell death & disease 11:832 PubMed33028809

2020

LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Tingzhuang Yi,Hongcheng Luo,Fengxue Qin,Qi Jiang,Shougao He,Tonghua Wang,Jianwei Su,Sien Song,Xiaoshan Qin,Yueqiu Qin,Xihan Zhou,Zansong Huang

View all publications

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重组Anti-Caspase-6/CASP-6抗体[EPR18043]

Anti-Caspase-6/CASP-6 antibody [EPR18043]

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (AB185645)