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重组RabMAb

重组Anti-Caspase-5抗体[EP876Y] (ab40887)

Submit a review Q&A (1)References (6)
Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] (ab40887)
  • Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (ab40887)
  • Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
  • Anti-Caspase-5 antibody [EP876Y] (ab40887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP876Y] to Caspase-5
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Caspase-5抗体[EP876Y]
    参阅全部 Caspase-5 一抗

  • 描述

    兔单克隆抗体[EP876Y] to Caspase-5

  • 宿主

    Rabbit

  • 特异性

    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.

  • 经测试应用

    适用于: IHC-P, IP, WBmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Caspase-5 aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照

    • IHC-P: Human breast carcinoma. WB: HT-1080 and HeLa cell lysates. IP: HeLa cell lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP876Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40887于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Mediator of programmed cell death (apoptosis).

  • 组织特异性

    Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • 翻译后修饰

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    图片

    • Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/1000 dilution + HT-1080 (Human fibrosarcoma epithelial cell) prepared in 1%SDS Hot lysis method whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking/Diluting Buffer and concentration:
      5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-5 antibody [EP876Y] (ab40887)

      Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Immunoprecipitation - Anti-Caspase-5 antibody [EP876Y] (ab40887)

      Purified ab40887 at 1/50 dilution (2µg) immunoprecipitating Caspase-5 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat shock (42? 1h) then recovery for 6h whole cell lysate 10µg
      Lane 2 (+): ab40887 + HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40887 in HeLa treated with heat shock (42? 1h) then recovery for 6h whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 47 kDa

    • Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Western blot - Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Anti-Caspase-5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      The cell lysates were prepared in 1%SDS Hot lysis method.

    • Anti-Caspase-5 antibody [EP876Y] (ab40887)
      Anti-Caspase-5 antibody [EP876Y] (ab40887)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (6)

    发表研究结果有使用 ab40887？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab40887 被引用在 6 文献中.

    • Wu Z  et al. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules 12:N/A (2022). PubMed: 36359010
    • Wang X  et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J 40:e108080 (2021). PubMed: 34747049
    • Zhang Z  et al. Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury. Cell Death Dis 9:983 (2018). PubMed: 30250284
    • Aydogan A  et al. Immunohistochemical expression of caspase-3, caspase-5, caspase-7 and apoptotic protease-activating factor-1 (APAF-1) in the liver and kidney of rats exposed to zoledronic acid (ZOL) and basic fibroblast growth factor (bFGF). Vet Q 34:137-42 (2014). PubMed: 24865775
    • Manchado E  et al. Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase. Cancer Cell 18:641-54 (2010). WB ; Mouse . PubMed: 21156286
    • Williams DS  et al. Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability. PLoS One 5:e16012 (2010). PubMed: 21209843

    客户评价及客户问答

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    Question

    I am interested in purchasing a selection of antibodies for the study of truncated variants of Bax, Caspase 5 and TGF beta receptor II. The following antibodies may be of use to me, as your datasheets indicate that the N-terminus has been used as the immunogen. However, I need to know which specific residues have been used in order to know whether the antibodies are going to be helpful. I wish to use the antibodies in Western Blot & IHC experiments on colon cancer cell lines. Bax ab15181 (ab33020, ab5714 & ab7977 all have specific immunogen peptide sequences supplied) Caspase 5 ab25899 ab40887 TGF beta receptor II ab28382 ab28383 I would also be interested in antibodies to the N-terminus of TAF1B, TCF7L2, IGF2R (ab26938 should be okay) and MSH3. Ideally I would like to order soon to have the antibodies for use by late January. Thankyou for you help with this Regards

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    Answer

    Thank you for your enquiry. I have contacted the originators of these antibodies and here is the sequence information I obtained. ab25899: This immunogen sequence is proprietary, but we can run any alignments with the sequence that you require. ab40887: The immunogen is 19aa long peptide that lies within the first 50 aas on N-termincal of human Caspase-5. ab28383/ab28382:LDTLVGKGRFAEVYKAKLKQN (these are the same antibody, one is prediluted and one is concentrated) ab15181: GSGEQPRGGGPTSSC Before buying any of these antibodies, I recommend that you check to make sure they are tested in Western blot and IHC and also make sure they have been tested in the sepcies of the sample you are using. We can only guarantee the antibodies if you use them as they are described on the data sheets. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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