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Cell Biology Apoptosis Intracellular Caspases etc Caspases
使用敲除细胞株进行验证重组RabMAb

重组Anti-Caspase-3抗体[E87] (ab32351)

  • Datasheet
  • SDS
Reviews (14)Q&A (4)References (205)

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Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
  • Immunoprecipitation - Anti-Caspase-3 antibody [E87] (ab32351)
  • Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)
  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
  • Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)
  • Anti-Caspase-3 antibody [E87] (ab32351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E87] to Caspase-3
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Biotin Carrier Free HRP PE

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概述

  • 产品名称

    Anti-Caspase-3抗体[E87]
    参阅全部 Caspase-3 一抗
  • 描述

    兔单克隆抗体[E87] to Caspase-3
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific for the pro form and the p17 cleaved form of human Caspase-3.
  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details
  • 种属反应性

    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide within Human Caspase-3 aa 50-150. The exact sequence is proprietary.
    Database link: P42574

  • 阳性对照

    • WB: Jurkat whole cell lysate (ab7899); Wild-type HAP1 whole cell lysate; Ramos and HEK-293 cell lysates. IHC-P: Human tonsil and cervical carcinoma tissue. ICC/IF: Jurkat cells and wild-type HAP1 cells. Flow Cyt (intra): HeLa and Ramos cells. IP: HeLa whole cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    E87
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • Biotin Anti-Caspase-3 antibody [E87] (ab195905)
    • Anti-Caspase-3 antibody [E87] - BSA and Azide free (ab197202)
  • Assay kits

    • Caspase-3 Assay Kit (Colorimetric) (ab39401)
    • Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Caspase-3 protein (BSA and azide free) (denatured) (ab180336)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32351于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (3)
Use a concentration of 1 µg/ml.

For unpurified, use 1/25 dilution.

Flow Cyt (Intra)
1/180 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (7)
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P (2)
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP
1/10 - 1/50.
说明
ICC/IF
Use a concentration of 1 µg/ml.

For unpurified, use 1/25 dilution.

Flow Cyt (Intra)
1/180 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP
1/10 - 1/50.

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 组织特异性

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性

    Belongs to the peptidase C14A family.
  • 翻译后修饰

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 836 Human
    • Omim: 600636 Human
    • SwissProt: P42574 Human
    • Unigene: 141125 Human
    • 别名

      • A830040C14Rik antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Casp3a antibody
      • Caspase 3 antibody
      • Caspase 3, apoptosis-related cysteine peptidase antibody
      • Caspase 3, apoptosis-related cysteine protease antibody
      • Caspase 3, apoptosis-related cysteine protease a antibody
      • Caspase-3 subunit p12 antibody
      • Caspase3 antibody
      • CC3 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • EC 3.4.22.56 antibody
      • ICE3 antibody
      • LICE antibody
      • mldy antibody
      • OTTHUMP00000165052 antibody
      • OTTHUMP00000165053 antibody
      • OTTHUMP00000165054 antibody
      • PARP cleavage protease antibody
      • Procaspase3 antibody
      • Protein Yama antibody
      • SCA 1 antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      • Yama protein antibody
      see all

    图片

    • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution

      Lane 1 : DMSO control wild-type HAP1 whole cell lysate
      Lane 2 : Staurosporine treated wild-type HAP1 whole cell lysate
      Lane 3 : DMSO control CASP3 knockout HAP1 whole cell lysate
      Lane 4 : Staurosporine treated CASP3 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 32 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab32351 observed at 31 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab32351 was shown to recognize Caspase 3 in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 Staurosporine Treated (CASP3) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HAP1 Staurosporine Treated (CASP3) knockout samples were subjected to SDS-PAGE. ab32351 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
      ab32351 staining Caspase-3 in wild-type Hap1 cells (top panel) and CASP3 knockout Hap1 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32351 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunohistochemical staining of paraffin embedded human tonsil with purified ab32351 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution (purified)

      Lane 1 : untreated Jurkat (human T cell leukemia cell line from peripheral blood)cell lysate
      Lane 2 : Jurkat treated with staurosporine

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 17,35 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)

      Immunofluorescence staining of Jurkat (human T cell leukemia cell line from peripheral blood) cells with purified ab32351 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32351 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)

      Unpurified ab32351, at a 1/25 dilution, staining Capase-3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution (purified)

      Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
      Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-Caspase-3 antibody [E87] (ab32351)
      Immunoprecipitation - Anti-Caspase-3 antibody [E87] (ab32351)

      ab32351 (purified) at 1/50 immunoprecipitating Cullin 1 in 10 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)
      Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)

      Overlay histogram showing Ramos (human Burkitt's lymphoma cell line) cells fixed in 4% PFA and stained with purified ab32351 at a dilution of 1 in 180 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). 

       

       

    • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
      Western blot - Anti-Caspase-3 antibody [E87] (ab32351)

      Carried out with unpurified antibody. Lane 1 = Caspase 3 protein (Active) (ab52314) 20 ng. Lane 2 = Caspase 9 protein (Active) (ab52203) 20 ng. Lane 3 = Extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with vehicle (ab136806) 20 ug. Lane 4 = Extract of HeLa cells treated with staurosporine (ab136806) 20 ug. SDS PAGE performed under reducing conditions (100 mM DTT Sample heated at 50°C). Primary : Lanes 1-4: Anti Caspase 3 antibody (ab32351) at 1:1000 dilution. Secondary : Lanes 1-4: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL for 10 min exposure. Blocking: in 5% Milk + PBS overnight at 4 C. Primary antibody: in 5% Milk + PBS for 2 hours at RT. Secondary antibody: in 5% Milk + PBS for 2 hours at RT. Predicted band size : 32 kDa and 17 kDa. Observed band size : 32 kDa and 17 kDa.

    • Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)
      Flow Cytometry (Intracellular) - Anti-Caspase-3 antibody [E87] (ab32351)

      Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurfied ab32351 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32351, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr®488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Anti-Caspase-3 antibody [E87] (ab32351)
      Anti-Caspase-3 antibody [E87] (ab32351)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (205)

    发表研究结果有使用 ab32351?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab32351 被引用在 205 文献中.

    • Jing C  et al. Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness. Theranostics 11:2655-2669 (2021). PubMed: 33456565
    • Liu C  et al. Cytoplasmic SHMT2 drives the progression and metastasis of colorectal cancer by inhibiting ß-catenin degradation. Theranostics 11:2966-2986 (2021). PubMed: 33456583
    • Gui C  et al. Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF-7 cell line. Int J Oncol 58:409-418 (2021). PubMed: 33469684
    • Zhao S  et al. Effects of Smad4 on the expression of caspase-3 and Bcl-2 in human gingival fibroblasts cultured on 3D PLGA scaffolds induced by compressive force. Int J Mol Med 47:N/A (2021). PubMed: 33495811
    • Haasler L  et al. The BH3 mimetic (±) gossypol induces ROS-independent apoptosis and mitochondrial dysfunction in human A375 melanoma cells in vitro. Arch Toxicol 95:1349-1365 (2021). PubMed: 33523262
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 18 Abreviews or Q&A

    Western blot abreview for Anti-Caspase-3 [E87] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (RAMOS cells)
    Specification
    RAMOS cells
    Treatment
    with or without TGFbeta (50pM, 24h)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 07 2014

    Western blot abreview for Anti-Caspase-3 antibody [E87]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    B16 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 15 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caspase-3 antibody [E87]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice B16 tumor cells)
    Permeabilization
    Yes - 0.25% Triton-X100 10 minutes
    Specification
    Mice B16 tumor cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 20 2017

    Western blot abreview for Anti-Caspase-3 antibody [E87]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (N87 cell line)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel)
    Loading amount
    800000 cells
    Specification
    N87 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 03 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody [E87]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.05% trypsin
    Sample
    Mouse Tissue sections (prostate)
    Specification
    prostate
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 05 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Caspase-3 antibody [E87]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Goat serum 5% +BSA1% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 23°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - triton 0.2%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Francoise Geffroy

    Verified customer

    提交于 Sep 16 2014

    Western blot abreview for Anti-Caspase-3 [E87] antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    African Green Monkey Cell lysate - whole cell (COS-1 cells)
    Specification
    COS-1 cells
    Treatment
    with or without TGFbeta (50pM, 24h)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 07 2014

    Western blot abreview for Anti-Caspase-3 [E87] antibody

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (NMuMG cells)
    Specification
    NMuMG cells
    Treatment
    with or without TGFbeta (50pM, 24h)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 07 2014

    Question

    Phone call enquiring after an antibody capable of detecting both the Caspase 3 pro form and the cleaved form of the protein.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 02 2012

    Answer

    This antibody will detect both the pro-form and p17 cleaved-form of Caspase-3 and has been shown to work well in Western blotting with Jurkat cell lysate.

    Read More

    Abcam Scientific Support

    回复于 Oct 02 2012

    Question

    Thank you for your kind reply.
    I ordered the product you suggested, and hope this work.
    This one is the first antibody, right? Could you also suggest me a secondary antibody?

    Thank you, and wish you all the best

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    The product I would like to suggest is the following:

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)

    https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab97051.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab97051.html).



    Read More

    Abcam Scientific Support

    回复于 Sep 06 2012

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