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Cell Biology Apoptosis Intracellular Caspases etc Caspases

Anti-Caspase-3抗体(ab4051)

  • Datasheet
  • SDS
Reviews (8)Q&A (17)References (248)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
  • Western blot - Anti-Caspase-3 antibody (ab4051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)

Key features and details

  • Rabbit polyclonal to Caspase-3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human Caspase-3 protein (BSA and azide free) (denatured) (ab180336)

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概述

  • 产品名称

    Anti-Caspase-3抗体
    参阅全部 Caspase-3 一抗
  • 描述

    兔多克隆抗体to Caspase-3
  • 宿主

    Rabbit
  • 特异性

    Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating two subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.

    This antibody reacts with the 32 kDa proenzyme and the 17 kDa active form of caspase 3.


     

  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-3 aa 167-175. Corresponding to the cleavage site of human caspase 3.
    Database link: P42574

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    Purified immunoglobulin fraction of rabbit antiserum against Caspase-3 containing sodium azide as a preservative.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

相关产品

  • Assay kits

    • Caspase-3 Assay Kit (Colorimetric) (ab39401)
    • Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Caspase-3 protein (BSA and azide free) (denatured) (ab180336)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4051于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IHC-P (7)
Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IHC-P
Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • 组织特异性

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性

    Belongs to the peptidase C14A family.
  • 翻译后修饰

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 836 Human
    • Omim: 600636 Human
    • SwissProt: P42574 Human
    • Unigene: 141125 Human
    • 别名

      • A830040C14Rik antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Casp3a antibody
      • Caspase 3 antibody
      • Caspase 3, apoptosis-related cysteine peptidase antibody
      • Caspase 3, apoptosis-related cysteine protease antibody
      • Caspase 3, apoptosis-related cysteine protease a antibody
      • Caspase-3 subunit p12 antibody
      • Caspase3 antibody
      • CC3 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • EC 3.4.22.56 antibody
      • ICE3 antibody
      • LICE antibody
      • mldy antibody
      • OTTHUMP00000165052 antibody
      • OTTHUMP00000165053 antibody
      • OTTHUMP00000165054 antibody
      • PARP cleavage protease antibody
      • Procaspase3 antibody
      • Protein Yama antibody
      • SCA 1 antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      • Yama protein antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)

      ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    • Western blot - Anti-Caspase-3 antibody (ab4051)
      Western blot - Anti-Caspase-3 antibody (ab4051)
      Anti-Caspase-3 antibody (ab4051) + Human Lung Cell Extract

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReview

      ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReview

      ab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (248)

    发表研究结果有使用 ab4051?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4051 被引用在 248 文献中.

    • Karabulut D  et al. Thymoquinone attenuates doxorubicin-cardiotoxicity in rats. J Biochem Mol Toxicol 35:e22618 (2021). PubMed: 32860490
    • Asci H  et al. Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity. Biotech Histochem 96:364-375 (2021). PubMed: 32921172
    • Lv YT  et al. Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis. Adipocyte 10:28-37 (2021). PubMed: 33393852
    • Xin Y  et al. Inhibition of miR-101-3p protects against sepsis-induced myocardial injury by inhibiting MAPK and NF-?B pathway activation via the upregulation of DUSP1. Int J Mol Med 47:N/A (2021). PubMed: 33448324
    • Jin Q  et al. Allisartan isoproxil attenuates oxidative stress and inflammation through the SIRT1/Nrf2/NF-?B signalling pathway in diabetic cardiomyopathy rats. Mol Med Rep 23:N/A (2021). PubMed: 33495841
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 25 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH = 6, citrate
    Permeabilization
    No
    Specification
    Brain cortex
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (bone graft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
    Permeabilization
    Yes - 0.05% triton-x100
    Specification
    bone graft
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 13 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA High pH
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Intestines)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Intestines
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 20 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (intestine)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    intestine
    Fixative
    10% NBF
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 05 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Caspase-3 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Gingiva)
    Permeabilization
    Yes - Triton X-100
    Specification
    Gingiva
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    提交于 Apr 25 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    None
    Sample
    Cow Tissue sections (Ovary)
    Specification
    Ovary
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 24 2014

    Question

    Customer inquiring about concentration of ab4051 lot XXXXXX.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Thank you for contacting Abcam.

    The concentration of ab4051 lot XXXXXX is XXX mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Nov 27 2012

    Question


    can it be shipped at room ?

    How long will it be valid after its opening? I am asking because i want to test 20 samples then after 8months test rest of samples..can i do that or the kit will be expired?

    What is the size of this kit?

    Read More

    Abcam community

    Verified customer

    Asked on May 12 2014

    Answer





    1. can it be shipped at room ? ab137994 is shipped on wet ice at 4 degrees C.


    2. How long will it be valid after its opening? I am asking because i want to test 20 samples then after 8months test rest of samples..can i do that or the kit will be expired? ==== It will probably be OK to store this kit for 8 months between tests. Please read the Protocol Booklet sections 9 and 10 on Assay Preparation and Reagent Preparation as specific storage requirements of prepared reagents are described. For example, only diluite enough 'Diluent N' required because once diluted it can only be stored for 1 month at 4 degrees C. Also, only use 1 vial of Standard with the first round of samples, so you can store the second vial for use in 8 months with the next round of samples. This is because once diluted, the Standard can only be stored for 30 days at -20 degrees C.


    3. What is the size of this kit? ==== This kit provides reagents sufficient for 96 tests, which is not the same thing as 96 samples. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. Review the protocol completely to confirm this kit meets your requirements.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    回复于 May 12 2014

    Question

    Hello,

    I would like to ask whether ab4051 is useful for the IHC-Fr detection (paraffin fixed formallin embded).

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2013

    Answer

    Thank you for your enquiry and your interest in our products.

    As the datasheet indicates ab4051 has been tested for both IHC-P (paraffin embedded formallin fixed tissues) and IHC-Fr (frozen tissues).

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Jan 24 2013

    1-10 of 25 Abreviews or Q&A

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