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重组RabMAb

重组Anti-Caspase-14抗体[EPR12927] (ab174847)

Reviews (1) Submit a question References (5)
Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)
  • Anti-Caspase-14 antibody [EPR12927] (ab174847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12927] to Caspase-14
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP

概述

  • 产品名称

    Anti-Caspase-14抗体[EPR12927]
    参阅全部 Caspase-14 一抗

  • 描述

    兔单克隆抗体[EPR12927] to Caspase-14

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • MCF7, ECV-304, HACAT and Human skin lysates; fetal skin tissue; Human skin tissue; pellet from fetal skin lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12927

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174847于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.

靶标

  • 功能

    Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.

  • 组织特异性

    Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.

  • 序列相似性

    Belongs to the peptidase C14A family.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apoptosis related cysteine protease antibody
      • CASP 14 antibody
      • CASP-14 antibody
      • CASP14 antibody
      • Caspase 14 apoptosis related cysteine protease antibody
      • Caspase 14 precursor antibody
      • Caspase-14 subunit p10 antibody
      • Caspase14 antibody
      • CASPE_HUMAN antibody
      • MGC119078 antibody
      • MGC119079 antibody
      • MICE antibody
      • Mini ICE antibody
      see all

    图片

    • Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      All lanes : Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : ECV-304 cell lysate
      Lane 3 : HACAT cell lysate
      Lane 4 : Human skin lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (ab174847)

      Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.

      -ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)

      Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (ab174847)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (ab174847)

      Intracellular flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14using ab174847 at a 1/10 dilution (red), or rabbit IgG negative control (green). 

       

       

    • Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)

      Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-Caspase-14 antibody [EPR12927] (ab174847)
      Anti-Caspase-14 antibody [EPR12927] (ab174847)

    文献 (5)

    发表研究结果有使用 ab174847？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab174847 被引用在 5 文献中.

    • Cui HS  et al. Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin. Int J Mol Sci 24:N/A (2023). PubMed: 37047109
    • Zuniga K  et al. Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels. Bioengineering (Basel) 9:N/A (2022). PubMed: 36290526
    • Cui HS  et al. Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars. Sci Rep 11:17296 (2021). PubMed: 34453089
    • Alkhanjaf AAM  et al. Moonlighting Proteins and Cardiopathy in the Spatial Response of MCF-7 Breast Cancer Cells to Tamoxifen. Proteomics Clin Appl 13:e1900029 (2019). PubMed: 31282103
    • Moriya C  et al. PRDM14 directly interacts with heat shock proteins HSP90a and glucose-regulated protein 78. Cancer Sci 109:373-383 (2018). PubMed: 29178343

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caspase-14 antibody [EPR12927]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocyte)
    Permeabilization
    No
    Specification
    Keratinocyte
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jul 15 2020

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