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Anti-Caspase-10/CASP-10抗体(ab2012)

Reviews (1)Q&A (4)References (3)
Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
  • Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)

Key features and details

  • Rabbit polyclonal to Caspase-10/CASP-10
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Caspase-10/CASP-10抗体
    参阅全部 Caspase-10/CASP-10 一抗

  • 描述

    兔多克隆抗体to Caspase-10/CASP-10

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human Caspase-10/CASP-10 aa 505-521 (C terminal).
    Sequence:

    ISAQTPRPPMRRWSSVS


    Database link: Q92851
    (Peptide available as ab8377)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HeLa whole cell lysate. ICC: HeLa cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2012于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
说明
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.

  • 组织特异性

    Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

  • 疾病相关

    Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.

  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.

  • 翻译后修饰

    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ALPS2 antibody
      • Apoptotic protease Mch-4 antibody
      • CASP 10 antibody
      • CASP-10 antibody
      • CASP10 antibody
      • CASPA_HUMAN antibody
      • Caspase 10 apoptosis related cysteine peptidase antibody
      • Caspase-10 antibody
      • Caspase-10 subunit p12 antibody
      • FADD like ICE2 antibody
      • Fas associated death domain protein antibody
      • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
      • FLICE 2 antibody
      • FLICE2 antibody
      • ICE like apoptotic protease 4 antibody
      • ICE-like apoptotic protease 4 antibody
      • Interleukin 1B converting enzyme 2 antibody
      • MCH 4 antibody
      see all

    图片

    • Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
      Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
      All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : K562 whole cell lysate
      Lane 5 : NIH3T3 whole cell lysate

      Predicted band size: 59 kDa
      Observed band size: 59 kDa

    • Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)
      Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)

      Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody (ab2012) at 5μg/ml.

    文献 (3)

    发表研究结果有使用 ab2012？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2012 被引用在 3 文献中.

    • Langford MP  et al. Multiple caspases mediate acute renal cell apoptosis induced by bacterial cell wall components. Ren Fail 33:192-206 (2011). PubMed: 21332342
    • Pagnan G  et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . PubMed: 19228726
    • Green KA  et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). PubMed: 15069074

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caspase-10/CASP-10 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (cultured hippocampal astrocytes)
    Specification
    cultured hippocampal astrocytes
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 08 2007

    Question

    I need to ask a same question that you were asked in 2002 November by someone. Question 2 Monday 18-November-2002 Has this antibody been tried on paraffin sections in immunohistochemistry? ANSWER As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC. still you did not try to apply on immunohistochemistry.

    Read More
    Answer

    Thank you for your interest in our products. Although it has been years since the last enquiry, this product has yet to be tested in immunohistochemistry. However, we have just bought a new facility in the UK, equipped with high tech machines, that will facilitate faster testing and imaging of products in IHC, WB, ELISA, FACS, and etc. Please rest assured that our goal is to have all products tested in at least IHC, WB, and ELISA but our facility is currently under staff, and trying to cope with all the requests that we had from customers as well as trying to maintain a constant release of new products. Please be patient with us until we can fully churn out more info for each product. If you need to use this product urgently, then may I encourage you make use of our Abreview system. We always encourage customers to send their results back to us, whether positive or negative. Each Abreview will be rewarded Abpoints, which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews Thank you for your feedback and for being our loyal customer.

    Read More

    Question

    We are in the process of publishing a paper containing data on caspase 10. To satisfy the reviewers we have been asked to repeat some of the experiments using a different caspase 10 antibody. We would like to use your antibody which recognises the N- terminus but this has not been tested in the mouse. I was wondering if it was possible to gain a small aliquote of this sample to test in exchange for our results.

    Read More
    Answer

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Question

    Has this antibody been tried on paraffin sections in immunohistochemistry?

    Read More
    Answer

    As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC.

    Read More

    Question

    will this recognize the mouse species Please email us with specifications for use in cell lines and westerns. Please also include the pricing for the antibody.

    Read More
    Answer

    Yes, anti-caspase-10 is mouse reactive. 3T3 cell lysate was tested by Western blot. $195 /100 µg + $25 for FedEx overnight shipping.

    Read More

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