为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cell Biology Apoptosis Intracellular Caspases etc Caspases

Anti-Caspase-10/CASP-10抗体(ab2012)

  • Datasheet
Reviews (1)Q&A (4)References (3)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
  • Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)

Key features and details

  • Rabbit polyclonal to Caspase-10/CASP-10
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Caspase-10/CASP-10抗体
    参阅全部 Caspase-10/CASP-10 一抗
  • 描述

    兔多克隆抗体to Caspase-10/CASP-10
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-10/CASP-10 aa 505-521 (C terminal).
    Sequence:

    ISAQTPRPPMRRWSSVS


    Database link: Q92851
    (Peptide available as ab8377)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HeLa whole cell lysate. ICC: HeLa cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    DEAE-Chromatography
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Assay kits

    • Caspase-10 (active) Staining Kit - Green Fluorescence (ab219937)
    • Caspase 10 Assay Kit (Colorimetric) (ab65663)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Caspase-10/CASP-10 protein (ab52080)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2012于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
说明
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.
  • 组织特异性

    Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • 疾病相关

    Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • 翻译后修饰

    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession Q92851 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 843 Human
    • Omim: 601762 Human
    • SwissProt: Q92851 Human
    • Unigene: 5353 Human
    • 别名

      • ALPS2 antibody
      • Apoptotic protease Mch-4 antibody
      • CASP 10 antibody
      • CASP-10 antibody
      • CASP10 antibody
      • CASPA_HUMAN antibody
      • Caspase 10 apoptosis related cysteine peptidase antibody
      • Caspase-10 antibody
      • Caspase-10 subunit p12 antibody
      • FADD like ICE2 antibody
      • Fas associated death domain protein antibody
      • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
      • FLICE 2 antibody
      • FLICE2 antibody
      • ICE like apoptotic protease 4 antibody
      • ICE-like apoptotic protease 4 antibody
      • Interleukin 1B converting enzyme 2 antibody
      • MCH 4 antibody
      see all

    图片

    • Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
      Western blot - Anti-Caspase-10/CASP-10 antibody (ab2012)
      All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : K562 whole cell lysate
      Lane 5 : NIH3T3 whole cell lysate

      Predicted band size: 59 kDa
      Observed band size: 59 kDa

    • Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)
      Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (ab2012)

      Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody (ab2012) at 5μg/ml.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab2012?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2012 被引用在 3 文献中.

    • Langford MP  et al. Multiple caspases mediate acute renal cell apoptosis induced by bacterial cell wall components. Ren Fail 33:192-206 (2011). PubMed: 21332342
    • Pagnan G  et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . PubMed: 19228726
    • Green KA  et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). PubMed: 15069074

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caspase-10/CASP-10 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (cultured hippocampal astrocytes)
    Specification
    cultured hippocampal astrocytes
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1%
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 08 2007

    Question

    I need to ask a same question that you were asked in 2002 November by someone. Question 2 Monday 18-November-2002 Has this antibody been tried on paraffin sections in immunohistochemistry? ANSWER As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC. still you did not try to apply on immunohistochemistry.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2007

    Answer

    Thank you for your interest in our products. Although it has been years since the last enquiry, this product has yet to be tested in immunohistochemistry. However, we have just bought a new facility in the UK, equipped with high tech machines, that will facilitate faster testing and imaging of products in IHC, WB, ELISA, FACS, and etc. Please rest assured that our goal is to have all products tested in at least IHC, WB, and ELISA but our facility is currently under staff, and trying to cope with all the requests that we had from customers as well as trying to maintain a constant release of new products. Please be patient with us until we can fully churn out more info for each product. If you need to use this product urgently, then may I encourage you make use of our Abreview system. We always encourage customers to send their results back to us, whether positive or negative. Each Abreview will be rewarded Abpoints, which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews Thank you for your feedback and for being our loyal customer.

    Read More

    Abcam Scientific Support

    回复于 Dec 20 2007

    Question

    We are in the process of publishing a paper containing data on caspase 10. To satisfy the reviewers we have been asked to repeat some of the experiments using a different caspase 10 antibody. We would like to use your antibody which recognises the N- terminus but this has not been tested in the mouse. I was wondering if it was possible to gain a small aliquote of this sample to test in exchange for our results.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2003

    Answer

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Abcam Scientific Support

    回复于 Oct 13 2003

    Question

    Has this antibody been tried on paraffin sections in immunohistochemistry?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2002

    Answer

    As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC.

    Read More

    Abcam Scientific Support

    回复于 Nov 18 2002

    Question

    will this recognize the mouse species Please email us with specifications for use in cell lines and westerns. Please also include the pricing for the antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 10 2001

    Answer

    Yes, anti-caspase-10 is mouse reactive. 3T3 cell lysate was tested by Western blot. $195 /100 µg + $25 for FedEx overnight shipping.

    Read More

    Abcam Scientific Support

    回复于 Feb 10 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有