Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Caspase-1抗体(ab62698)

Reviews (1)Q&A (1)References (24)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
  • Western blot - Anti-Caspase-1 antibody (ab62698)

Key features and details

  • Rabbit polyclonal to Caspase-1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Caspase-1抗体
    参阅全部 Caspase-1 一抗

  • 描述

    兔多克隆抗体to Caspase-1

  • 宿主

    Rabbit

  • 特异性

    ab62698 recognizes Caspase-1 subunit p10.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • 阳性对照

    • Human brain tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab62698于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/1000.
IHC-P
1/50 - 1/100.
说明
WB
1/500 - 1/1000.
IHC-P
1/50 - 1/100.

靶标

  • 功能

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

  • 组织特异性

    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • 翻译后修饰

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CASP-1 antibody
      • CASP1 antibody
      • CASP1_HUMAN antibody
      • Caspase 1 antibody
      • Caspase-1 subunit p10 antibody
      • ICE antibody
      • IL-1 beta-converting enzyme antibody
      • IL-1BC antibody
      • IL1 beta converting enzyme antibody
      • IL1B convertase antibody
      • Interleukin 1 beta convertase antibody
      • Interleukin 1B converting enzyme antibody
      • Interleukin-1 beta convertase antibody
      • Interleukin-1 beta-converting enzyme antibody
      • p45 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)

      ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

    • Western blot - Anti-Caspase-1 antibody (ab62698)
      Western blot - Anti-Caspase-1 antibody (ab62698)
      All lanes : Anti-Caspase-1 antibody (ab62698)

      Lane 1 : 293 cell extract
      Lane 2 : MCF-7 cell extract
      Lane 3 : HeLa cell extract
      Lane 4 : 293 cell extract with blocking peptide

      Observed band size: 29 kDa why is the actual band size different from the predicted?

    文献 (24)

    发表研究结果有使用 ab62698？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab62698 被引用在 24 文献中.

    • Zhu QM  et al. A potential immunotherapy target for breast cancer: parenchymal and immune-stromal expression of the NLRP3 inflammasome pathway. BMC Cancer 23:1163 (2023). PubMed: 38031068
    • Chen X  et al. Mechanism of Baicalein in Brain Injury After Intracerebral Hemorrhage by Inhibiting the ROS/NLRP3 Inflammasome Pathway. Inflammation 45:590-602 (2022). PubMed: 34625906
    • Liu L  et al. Ubiquitin-specific protease 8 inhibits lipopolysaccharide-triggered pyroptosis of human bronchial epithelial cells by regulating PI3K/AKT and NF-?B pathways. Allergol Immunopathol (Madr) 50:96-103 (2022). PubMed: 35257552
    • Pu J  et al. Immunohistochemical analysis of pyroptosis-related protein expression in IgG4-related sialadenitis. J Oral Pathol Med 51:388-394 (2022). PubMed: 35218237
    • Zha S  et al. Topical Simvastatin Improves Lesions of Diffuse Normolipemic Plane Xanthoma by Inhibiting Foam Cell Pyroptosis. Front Immunol 13:865704 (2022). PubMed: 35619689
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Caspase-1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (carotid artery)
    Permeabilization
    No
    Specification
    carotid artery
    Blocking step
    Protein block serum-free (Dako) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 17 2019

    Question

    I am interested in your product anti-caspase 1 antibody (ab62698). Can you give me some additionnal information? Is there BSA present in the disolving buffer? Does the antibody recognizes the active form of human/mouse caspase 1? Is there literature available on the usage of this antibody in ELISA and/or IP?  

    Read More
    Answer

    Thank you for your inquiry. I can confirm that there is no BSA in the buffer and that the antibody recognizes Caspase-1 subunit p10.  There are no references citing the usage of this antibody in ELISA or IP.  As far as we're aware, this antibody has only been tested in WB, ELISA and IHC-P and not yet in IP.  Since this antibody is not yet tested in IP, I can offer a discount off a future purchase if you buy ab62698 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab62698 in IP.  I will then send a discount code. This code must be issued before purchasing ab62698 so please wait for my reply before ordering. 2. Purchase ab62698 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab62698 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.    

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com