Anti-Caspase-1抗体

• WB

Unknown

Western blot - Anti-Caspase-1 antibody (AB138483)

The predicted molecular weight of Caspase 1 is 45 kDa (SwissProt), however we expect to observe a banding pattern around 50 kDa.

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138483 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Caspase-1 antibody (ab138483) at 1 µg/mL

All lanes:

Spleen (Mouse) Tissue Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 51 kDa,74 kDa

true

Exposure time: 20min

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (AB138483)

IHC image of Caspase-1 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138483, 1μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

CiteAb

Western blot - Anti-Caspase-1 antibody (AB138483)

Caspase-1 western blotting using Anti-Caspase-1 antibody ab138483. Publication image and figure legend from Liu, Y., Wang, K., et al., 2023, Food Sci Nutr, Pubmed 37324843.

Mulberry extract inhibits the expression of NLRP3 in foam cells via MAPK signaling pathway. Foam cells were induced by treating RAW264.7 macrophages with 50 μg/mL ox-LDL for 24 hours. The cells were then given mulberry extract at low (5 mg/mL) or high concentration (10 mg/mL) as indicated. Following foam cell features were monitored. (a). Intracellular lipid level measured by Oil Red O staining. (b). Cholesterol ester content. (TC, total cholesterol; FC, free cholesterol; CE, cholesterol ester) qualified by cellular cholesterol assay of foam cells with or without treatment of mulberry extract as indicated. (c). Induced foam cells were treated with different concentrations of mulberry extract (0, 1, 5, 10 mg/mL) for 24 h and protein levels of NLRP3, caspase-1, ASC, and IL-1 β were determined by western blotting. (d) The protein levels of NLRP3, ASC, p-p38, p38 MAPK, and IL-1β protein levels of cells undergoing indicated treatments were determined by western blotting. Graph bars represent qualification of flow cytometry results (Data shown are mean ± SEM. Statistical significance was determined by two-way ANOVA with correction for multiple comparisons. *p < < .01, **p < < .001, ***p < < .0001, ##p < < .01 and ###p < < .001.

false

• WB

CiteAb

Western blot - Anti-Caspase-1 antibody (AB138483)

Caspase-1 western blotting using Anti-Caspase-1 antibody ab138483. Publication image and figure legend from Xiao, C., Zhao, H., et al., 2020, Front Physiol, Pubmed 32903383.

Tisp40 knockout protects against NLRP3 inflammasome activation in I/R-induced kidney. (A) Representative photomicrographs of tubular cell injury in mouse kidney tissue sections with HE staining and representative photomicrographs of pro- IL-1β, IL-1β, and IL-18 expression in mouse kidney tissue sections by immunohistochemistry, 400x, scale bar = 20 μm. (B) Statistical quantification analysis showed the injury score of HE staining in the kidney tissues (C,D,E) Statistical analysis showed the positive area of pro- IL-1β, IL-1β, and IL-18 in the kidney tissues. (F-J) The expression of NLRP3 and caspase-1 were analyzed by qRT-PCR and Western blot. Data are expressed as the mean ± SD. n = 6 per group. *p < < 0.05 vs. corresponding sham-operation groups (Sham). ^∧P > 0.05 vs. Tisp40^+/+ in sham groups. #p < < 0.05, δP > 0.05 vs. the group of I/R-induced Tisp40+/+ mice. one-way ANOVA.

false

• WB

CiteAb

Western blot - Anti-Caspase-1 antibody (AB138483)

Caspase-1 western blotting using Anti-Caspase-1 antibody ab138483. Publication image and figure legend from Xiao, C., Zhao, H., et al., 2020, Front Physiol, Pubmed 32903383.

Tisp40 enhanced NLRP3 inflammasome activation in cultured OGD/R-stimulated TCMK-1 cells. (A) The LDH release was detected by activity assays. IL-1β (B) and IL-18 (C) contents were measured by using ELISA kits. (D-H) The expression of NLRP3 and caspase-1 were analyzed by qRT-PCR and western blot. Data are expressed as the mean ± SD. n = 5 per group. *p < < 0.05 vs. corresponding control groups (Con). ^P > 0.05 vs. TCMK-1/vector in con group. #p < < 0.05 vs. the group of TCMK-1/vector cells induced by OGD/R, one-way ANOVA.

false