Anti-CASK抗体(ab3383)
Key features and details
- Rabbit polyclonal to CASK
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CASK抗体
参阅全部 CASK 一抗 -
描述
兔多克隆抗体to CASK -
宿主
Rabbit -
特异性
By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CASK aa 578-592.
Sequence:CERDSPSTSRQSPAN
(Peptide available asab4961) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
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| 说明 |
|---|
|
ICC
Use a concentration of 2 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. |
靶标
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功能
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. -
组织特异性
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver. -
疾病相关
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation. -
序列相似性
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
结构域
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2. -
细胞定位
Nucleus. Cytoplasm. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8573 Human
- Entrez Gene: 12361 Mouse
- Omim: 300172 Human
- SwissProt: O14936 Human
- SwissProt: O70589 Mouse
- Unigene: 495984 Human
- Unigene: 327591 Mouse
- Unigene: 474948 Mouse
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别名
- CAGH39 antibody
- Caki antibody
- Calcium/calmodulin dependent serine protein kinase antibody
see all
图片
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Immunocytochemistry - Anti-CASK antibody (ab3383)
Immunofluorescence analysis of CASK in U-87 MG cells using ab3383. The cells were fixed with 4% paraformaldehyde permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3383 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-CASK antibody (ab3383)Western blot detection of CASK on AtT20 cell lysate using ab3383.
数据表及文件
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Datasheet download
文献 (2)
ab3383 被引用在 2 文献中.
- Xiao X et al. N-wasp is required for structural integrity of the blood-testis barrier. PLoS Genet 10:e1004447 (2014). PubMed: 24967734
- Ojeh N et al. The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. J Cell Sci 121:2705-17 (2008). IHC ; Mouse, Rat . PubMed: 18664494
