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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-CASK抗体(ab3383)

  • Datasheet
Submit a review Submit a question References (2)

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Immunocytochemistry - Anti-CASK antibody (ab3383)
  • Western blot - Anti-CASK antibody (ab3383)

Key features and details

  • Rabbit polyclonal to CASK
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-CASK antibody [K56A/50] (ab252540)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-CASK抗体
    参阅全部 CASK 一抗
  • 描述

    兔多克隆抗体to CASK
  • 宿主

    Rabbit
  • 特异性

    By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus.
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human CASK aa 578-592.
    Sequence:

    CERDSPSTSRQSPAN


    (Peptide available as ab4961)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CASK protein (ab131707)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3383于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use a concentration of 2 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
说明
ICC
Use a concentration of 2 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.

靶标

  • 功能

    Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
  • 组织特异性

    Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
  • 疾病相关

    Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
    Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
  • 序列相似性

    In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 结构域

    The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
    The protein kinase domain mediates the interaction with FCHSD2.
  • 细胞定位

    Nucleus. Cytoplasm. Cell membrane.
  • Target information above from: UniProt accession O14936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8573 Human
    • Entrez Gene: 12361 Mouse
    • Omim: 300172 Human
    • SwissProt: O14936 Human
    • SwissProt: O70589 Mouse
    • Unigene: 495984 Human
    • Unigene: 327591 Mouse
    • Unigene: 474948 Mouse
    • 别名

      • CAGH39 antibody
      • Caki antibody
      • Calcium/calmodulin dependent serine protein kinase antibody
      • Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
      • Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody
      • Calcium/calmodulin-dependent serine protein kinase antibody
      • CAMGUK antibody
      • CAMGUK protein antibody
      • CAMGUK, drosophila, homolog of antibody
      • casK antibody
      • CMG antibody
      • CSKP_HUMAN antibody
      • DXPri1 antibody
      • DXRib1 antibody
      • FGS4 antibody
      • FLJ22219 antibody
      • FLJ31914 antibody
      • hCASK antibody
      • LIN 2 antibody
      • Lin 2 homolog antibody
      • LIN2 antibody
      • Lin2 homolog antibody
      • MICPCH antibody
      • MRXSNA antibody
      • Pals3 antibody
      • Peripheral plasma membrane protein CASK antibody
      • Protein lin-2 homolog antibody
      • TNRC8 antibody
      • Trinucleotide repeat containing 8 antibody
      • Vertebtate LIN2 homolog antibody
      see all

    图片

    • Immunocytochemistry - Anti-CASK antibody (ab3383)
      Immunocytochemistry - Anti-CASK antibody (ab3383)

      Immunofluorescence analysis of CASK in U-87 MG cells using ab3383. The cells were fixed with 4% paraformaldehyde permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3383 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.

    • Western blot - Anti-CASK antibody (ab3383)
      Western blot - Anti-CASK antibody (ab3383)

      Western blot detection of CASK on AtT20 cell lysate using ab3383.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab3383?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3383 被引用在 2 文献中.

    • Xiao X  et al. N-wasp is required for structural integrity of the blood-testis barrier. PLoS Genet 10:e1004447 (2014). PubMed: 24967734
    • Ojeh N  et al. The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. J Cell Sci 121:2705-17 (2008). IHC ; Mouse, Rat . PubMed: 18664494

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