重组Anti-Casein Kinase 2 beta抗体[EPR1994] (ab133576)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Casein Kinase 2 beta抗体[EPR1994]
参阅全部 Casein Kinase 2 beta 一抗 -
描述
兔单克隆抗体[EPR1994] to Casein Kinase 2 beta -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human breast tissue; HepG2, HeLa, SH SY5Y and HUVEC cell lysates
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.94 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1994 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use a HRP/AP polymerized secondary antibody. |
|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
|
说明 |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa). |
靶标
-
功能
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
序列相似性
Belongs to the casein kinase 2 subunit beta family. -
翻译后修饰
Phosphorylated by alpha subunit. - Information by UniProt
-
数据库链接
- Entrez Gene: 1460 Human
- Entrez Gene: 13001 Mouse
- Entrez Gene: 81650 Rat
- Omim: 115441 Human
- SwissProt: P67870 Human
- SwissProt: P67871 Mouse
- SwissProt: P67874 Rat
- Unigene: 73527 Human
see all -
别名
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
图片
-
All lanes : Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)
Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab133576 被引用在 3 文献中.
- Kim JM et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun 11:2289 (2020). PubMed: 32385263
- Beale AD et al. Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. J Biol Rhythms 34:144-153 (2019). PubMed: 30898060
- Miao Z et al. Comparative proteomic profile of the human placenta in normal and fetal growth restriction subjects. Cell Physiol Biochem 34:1701-10 (2014). PubMed: 25401993