重组Anti-Casein Kinase 2 beta抗体[EP1995Y] (ab76025)
![Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.cn/ps/products/76/ab76025/Images/ab76025-91330-anti-casein-kinase-2-beta-antibody-ep1995y-western-blot.gif "Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Horse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Casein Kinase 2 beta抗体[EP1995Y]
参阅全部 Casein Kinase 2 beta 一抗 -
描述
兔单克隆抗体[EP1995Y] to Casein Kinase 2 beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Horse, Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1995Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76025于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| ICC/IF |
1/100 - 1/250.
|
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| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
序列相似性
Belongs to the casein kinase 2 subunit beta family. -
翻译后修饰
Phosphorylated by alpha subunit. - Information by UniProt
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数据库链接
- Entrez Gene: 1460 Human
- Entrez Gene: 13001 Mouse
- Entrez Gene: 81650 Rat
- Omim: 115441 Human
- SwissProt: P67870 Human
- SwissProt: P67871 Mouse
- SwissProt: P67874 Rat
- Unigene: 73527 Human
see all -
别名
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
图片
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Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.cn/ps/products/76/ab76025/Images/ab76025-91329-anti-casein-kinase-2-beta-antibody-ep1995y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.cn/ps/products/76/ab76025/Images/ab76025-91328-anti-casein-kinase-2-beta-antibody-ep1995y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.cn/ps/products/76/ab76025/Images/ab76025-91327-anti-casein-kinase-2-beta-antibody-ep1995y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.cn/ps/products/76/ab76025/Images/ab76025-6-benefits-of-recombinant-antibodies.png)
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab76025 被引用在 21 文献中.
- Park K et al. ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. Nucleic Acids Res 50:3835-3851 (2022). PubMed: 35325203
- Asif M et al. De novo variants of CSNK2B cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway. HGG Adv 3:100111 (2022). PubMed: 35571680
- Yang W et al. Protein Kinase CK2 Controls CD8+ T Cell Effector and Memory Function during Infection. J Immunol 209:896-906 (2022). PubMed: 35914835
- Bartlett AP et al. Establishment and characterization of equine mammary organoids using a method translatable to other non-traditional model species. Development 149:N/A (2022). PubMed: 35297994
- Schwein PA et al. Writing and Erasing O-GlcNAc on Casein Kinase 2 Alpha Alters the Phosphoproteome. ACS Chem Biol 17:1111-1121 (2022). PubMed: 35467332