Anti-Casein Kinase 1 delta/CSNK1D抗体(ab48031)
Key features and details
- Goat polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Casein Kinase 1 delta/CSNK1D抗体
参阅全部 Casein Kinase 1 delta/CSNK1D 一抗 -
描述
山羊多克隆抗体to Casein Kinase 1 delta/CSNK1D -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta/CSNK1D.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
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免疫原
Synthetic peptide corresponding to Human Casein Kinase 1 delta/CSNK1D aa 310-324 (internal sequence).
Sequence:DREERLRHSRNPATR
Database link: P48730 -
阳性对照
- WB: HEK 293 cell lysate. ICC/UF U251 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48031于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
| ICC/IF |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
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ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
组织特异性
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
疾病相关
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
序列相似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on serine and threonine residues. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 407122 Cow
- Entrez Gene: 1453 Human
- Entrez Gene: 104318 Mouse
- Entrez Gene: 64462 Rat
- Omim: 600864 Human
- SwissProt: P35508 Cow
- SwissProt: P48730 Human
- SwissProt: Q9DC28 Mouse
see all -
别名
- Casein Kinase 1 delta antibody
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
see all
图片
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Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling Casein Kinase 1 delta/CSNK1D with ab48031 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
数据表及文件
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Datasheet download
文献 (3)
ab48031 被引用在 3 文献中.
- Vena F et al. Targeting Casein Kinase 1 Delta Sensitizes Pancreatic and Bladder Cancer Cells to Gemcitabine Treatment by Upregulating Deoxycytidine Kinase. Mol Cancer Ther 19:1623-1635 (2020). PubMed: 32430484
- Zhou A et al. Nuclear GSK3ß promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitylation by USP22. Nat Cell Biol 18:954-966 (2016). PubMed: 27501329
- Zyss D et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). PubMed: 22123863
