Anti-Casein Kinase 1 alpha (phospho T321)抗体(ab63559)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Casein Kinase 1 alpha (phospho T321)抗体
参阅全部 Casein Kinase 1 alpha 一抗 -
描述
兔多克隆抗体to Casein Kinase 1 alpha (phospho T321) -
宿主
Rabbit -
特异性
Detects Casein Kinase I alpha only when phosphorylated at threonine 321. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Casein Kinase 1 alpha aa 319-323 (phospho T321).
Sequence:A-Q-Tp-P-T
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阳性对照
- HuvEc cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63559于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. -
序列相似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 1452 Human
- Entrez Gene: 93687 Mouse
- Entrez Gene: 113927 Rat
- Omim: 600505 Human
- SwissProt: P48729 Human
- SwissProt: Q8BK63 Mouse
- SwissProt: P97633 Rat
- Unigene: 529862 Human
see all -
别名
- Casein kinase 1 alpha 1 antibody
- Casein kinase I isoform alpha antibody
- CK1 antibody
see all
图片
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Western blot - Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559)All lanes : Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559) at 1/500 dilution
Lane 1 : extracts from HuvEc cells
Lane 2 : extracts from HuvEc cells with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab63559 尚未被引用在任何文献中。