Anti-Carma 1/CARD11抗体(ab115471)
Key features and details
- Goat polyclonal to Carma 1/CARD11
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Carma 1/CARD11抗体
参阅全部 Carma 1/CARD11 一抗 -
描述
山羊多克隆抗体to Carma 1/CARD11 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human Carma 1/CARD11 aa 1100-1200 (C terminal) (Cysteine residue).
-
阳性对照
- Human spleen tissue; Jurkat cell lysate. The pattern of staining with this antibody is similar to that observed with ab189167 and ab113409 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115471于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 133 kDa.
|
|
| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 133 kDa. |
|
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. -
组织特异性
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells. -
序列相似性
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain. -
翻译后修饰
Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling. -
细胞定位
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
-
数据库链接
- Entrez Gene: 84433 Human
- Entrez Gene: 108723 Mouse
- Entrez Gene: 304314 Rat
- Omim: 607210 Human
- SwissProt: Q9BXL7 Human
- SwissProt: Q8CIS0 Mouse
- Unigene: 648101 Human
- Unigene: 46187 Mouse
-
别名
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
see all
图片
-
Western blot - Anti-Carma 1/CARD11 antibody (ab115471)Anti-Carma 1/CARD11 antibody (ab115471) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Primary incubation: 1 hour -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab115471)ab115471 at 2ug/ml staining Carma/CARD11 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab115471 尚未被引用在任何文献中。