Anti-Cardiac Troponin I (phospho T143)抗体(ab58546)
Key features and details
- Rabbit polyclonal to Cardiac Troponin I (phospho T143)
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-Cardiac Troponin I (phospho T143)抗体
参阅全部 Cardiac Troponin I 一抗 -
描述
兔多克隆抗体to Cardiac Troponin I (phospho T143) -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide corresponding to Human Cardiac Troponin I aa 100-200 (phospho T143).
Database link: P19429 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). |
|
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
疾病相关
Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Belongs to the troponin I family. - Information by UniProt
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数据库链接
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 100049696 Pig
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
- SwissProt: P19429 Human
- SwissProt: P48787 Mouse
- SwissProt: P23693 Rat
see all -
别名
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
see all
图片
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Western blot - Anti-cardiac Troponin I (phospho T143) antibody (ab58546)All lanes : Anti-Cardiac Troponin I (phospho T143) antibody (ab58546) at 1/500 dilution
Lane 1 : Mouse heart extract without immunizing peptide
Lane 2 : Mouse heart extract with immunizing peptide
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab58546 被引用在 8 文献中.
- Greenman AC et al. Increased myofilament calcium sensitivity is associated with decreased cardiac troponin I phosphorylation in the diabetic rat heart. Exp Physiol 106:2235-2247 (2021). PubMed: 34605091
- Ravichandran VS et al. Cardiac contractile dysfunction and protein kinase C-mediated myofilament phosphorylation in disease and aging. J Gen Physiol 151:1070-1080 (2019). PubMed: 31366607
- Gao R et al. R5 HIV-1 gp120 Activates p38 MAPK to Induce Rat Cardiomyocyte Injury by the CCR5 Coreceptor. Pathobiology 86:274-284 (2019). PubMed: 31574524
- Parikh VN et al. Apelin and APJ orchestrate complex tissue-specific control of cardiomyocyte hypertrophy and contractility in the hypertrophy-heart failure transition. Am J Physiol Heart Circ Physiol 315:H348-H356 (2018). PubMed: 29775410
- Wu SC et al. Nuclear localization of a1A-adrenergic receptors is required for signaling in cardiac myocytes: an “inside-out” a1-AR signaling pathway. J Am Heart Assoc 3:e000145 (2014). PubMed: 24772522
- Wang H et al. TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I. Braz J Med Biol Res 46:128-37 (2013). PubMed: 23369981
- Boontje NM et al. Enhanced myofilament responsiveness upon ß-adrenergic stimulation in post-infarct remodeled myocardium. J Mol Cell Cardiol 50:487-99 (2011). WB ; Pig . PubMed: 21156182
- Ladage D et al. Inhibition of PKCa/ß with ruboxistaurin antagonizes heart failure in pigs after myocardial infarction injury. Circ Res 109:1396-400 (2011). WB ; Pig . PubMed: 21998327