重组Anti-CARD9抗体[EPR6489] (ab133560)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6489] to CARD9
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CARD9抗体[EPR6489]
参阅全部 CARD9 一抗 -
描述
兔单克隆抗体[EPR6489] to CARD9 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CARD9 aa 450-550. The exact sequence is proprietary.
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阳性对照
- WB: K562, U937, HL60, HepG2, and THP1 cell lysates IP: K-562 whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.12 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6489 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133560于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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IP |
1/30.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
IP
1/30. |
靶标
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功能
Activates NF-kappa-B via BCL10. -
组织特异性
Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain. -
疾病相关
Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. -
序列相似性
Contains 1 CARD domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 64170 Human
- Omim: 607212 Human
- SwissProt: Q9H257 Human
- Unigene: 694071 Human
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别名
- CANDF2 antibody
- CARD9 antibody
- CARD9_HUMAN antibody
see all
图片
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All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution
Lane 1 : THP-1(Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 52-62 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
293T, A549 and NCI-H1299 are low expressors (PMID:?32164050, PMID:?33558980).
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All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/5000 dilution (Purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 52-62 kDa why is the actual band size different from the predicted?This antibody recognizes 2 isforms
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CARD9 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 µg with ab133560 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab133560 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 μg
Lane 2: ab133560 IP in K-562 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133560 in K-562 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution (Unpurified)
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133560 被引用在 2 文献中.
- Kan B et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). PubMed: 30446641
- Drummond RA et al. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11:e1005293 (2015). Flow Cyt ; Human . PubMed: 26679537