Anti-Carboxypeptidase Y抗体(ab181691)
Key features and details
- Rabbit polyclonal to Carboxypeptidase Y
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-Carboxypeptidase Y抗体
参阅全部 Carboxypeptidase Y 一抗 -
描述
兔多克隆抗体to Carboxypeptidase Y -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Carboxypeptidase Y aa 112-532.
Sequence:KIKDPKILGIDPNVTQYTGYLDVEDEDKHFFFWTFESRNDPAKDPVILWL NGGPGCSSLTGLFFELGPSSIGPDLKPIGNPYSWNSNATVIFLDQPVNVG FSYSGSSGVSNTVAAGKDVYNFLELFFDQFPEYVNKGQDFHIAGESYAGH YIPVFASEILSHKDRNFNLTSVLIGNGLTDPLTQYNYYEPMACGEGGEPS VLPSEECSAMEDSLERCLGLIESCYDSQSVWSCVPATIYCNNAQLAPYQR TGRNVYDIRKDCEGGNLCYPTLQDIDDYLNQDYVKEAVGAEVDHYESCNF DINRNFLFAGDWMKPYHTAVTDLLNQDLPILVYAGDKDFICNWLGNKAWT DVLPWKYDEEFASQKVRNWTASITDEVAGEVKSYKHFTYLRVFNGGHMVP FDVPENALSMVNEWIHGGFS L
Database link: P00729 -
阳性对照
- S. cerevisiae Carboxypeptidase Y protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.27% Tripotassium orthophosphate, 0.88% Sodium chloride
No stabilizer. -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
ab181691 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified S. cerevisiae Carboxypeptidase Y. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 60 kDa).
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. -
序列相似性
Belongs to the peptidase S10 family. -
翻译后修饰
Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa). -
细胞定位
Vacuole. Lysosome-like vacuoles. - Information by UniProt
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数据库链接
- Entrez Gene: 855343 Saccharomyces cerevisiae
- SwissProt: P00729 Saccharomyces cerevisiae
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别名
- carboxypeptidase C PRC1 antibody
- Carboxypeptidase Y antibody
- Carboxypeptidase YSCY antibody
see all
图片
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Anti-Carboxypeptidase Y antibody (ab181691) at 1/1000 dilution + S. cerevisiae Carboxypeptidase Y at 0.05 µg
Secondary
Peroxidase rabbit at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (1)
ab181691 被引用在 1 文献中.
- Vignogna RC et al. Evolutionary rescue of phosphomannomutase deficiency in yeast models of human disease. Elife 11:N/A (2022). PubMed: 36214454