重组Anti-Carbonic Anhydrase 3/CA3抗体[EPR13425] (ab181358)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Carbonic Anhydrase 3/CA3抗体[EPR13425]
参阅全部 Carbonic Anhydrase 3/CA3 一抗 -
描述
兔单克隆抗体[EPR13425] to Carbonic Anhydrase 3/CA3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Carbonic Anhydrase 3/CA3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P07451 -
阳性对照
- Human skeletal muscle, rat muscle, and mouse muscle lysates, Human skeletal muscle tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13425 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181358于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Reversible hydration of carbon dioxide. -
组织特异性
Muscle specific. -
序列相似性
Belongs to the alpha-carbonic anhydrase family. -
发展阶段
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
翻译后修饰
S-glutathionylated in hepatocytes under oxidative stress. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 761 Human
- Entrez Gene: 12350 Mouse
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: P07451 Human
- SwissProt: P16015 Mouse
- SwissProt: P14141 Rat
- Unigene: 82129 Human
see all -
别名
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase 3/CA3 with ab181358 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Rat muscle lysates
Lane 3 : Mouse muscle lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab181358 被引用在 1 文献中.
- Bell-Temin H et al. Measuring biological age in mice using differential mass spectrometry. Aging (Albany NY) 11:1045-1061 (2019). PubMed: 30745468