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重组RabMAb

重组Anti-Carbonic Anhydrase 1/CA1抗体[EPR5193] (ab108367)

Reviews (2) Submit a question References (10)
Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
  • Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
  • Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
  • Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human, Zebrafish

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Carbonic Anhydrase 1/CA1抗体[EPR5193]
    参阅全部 Carbonic Anhydrase 1/CA1 一抗

  • 描述

    兔单克隆抗体[EPR5193] to Carbonic Anhydrase 1/CA1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt (Intra)more details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat, Human, Zebrafish

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: TF-1, Mouse spleen lysates, Rat brain lysates, Rat spleen lysates, Zebrafish eye lysates, Mouse kidney lysates, HEL, K-562 whole cell lysates Flow Cyt (intra): K-562 cells IHC-Fr: Zebrafish retina tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR5193

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108367于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
Flow Cyt (Intra)
1/60.
说明
WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
Flow Cyt (Intra)
1/60.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

图片

  • Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution

    Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat spleen lysates
    Lane 5 : Zebrafish eye lysates
    Lane 6 : Mouse kidney lysates
    Lane 7 : HEL (Human Erythroleukemia erythroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)

    Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Carbonic Anhydrase I/CA1 with Purified ab108367 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : TF1 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa

  • Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
    Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)

文献 (10)

发表研究结果有使用 ab108367？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108367 被引用在 10 文献中.

  • Cheng K  et al. Upregulation of carbonic anhydrase 1 beneficial for depressive disorder. Acta Neuropathol Commun 11:59 (2023). PubMed: 37013604
  • Chen X  et al. Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice. Diabetes 71:795-811 (2022). PubMed: 35043173
  • Werning M  et al. A Potential Citrate Shunt in Erythrocytes of PKAN Patients Caused by Mutations in Pantothenate Kinase 2. Biomolecules 12:N/A (2022). PubMed: 35204826
  • Boulet C  et al. Red Blood Cell BCL-xL Is Required for Plasmodium falciparum Survival: Insights into Host-Directed Malaria Therapies. Microorganisms 10:N/A (2022). PubMed: 35456874
  • Gu W  et al. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell 29:101-115.e10 (2022). PubMed: 34582804
  • Werning M  et al. PKAN neurodegeneration and residual PANK2 activities in patient erythrocytes. Ann Clin Transl Neurol 7:1340-1351 (2020). PubMed: 32705819
  • Yuan L  et al. Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis. Front Pharmacol 10:766 (2019). PubMed: 31354482
  • Kankeu C  et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). PubMed: 29770421
  • Liu X  et al. Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS. Int J Mol Sci 17:N/A (2016). PubMed: 27809276
  • MacDonald RB  et al. Müller glia provide essential tensile strength to the developing retina. J Cell Biol 210:1075-83 (2015). PubMed: 26416961

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Carbonic Anhydrase I/CA1 antibody [EPR5193]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
10 µg
Treatment
CA1-3xflag transfected
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 30 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Carbonic Anhydrase I antibody [EPR5193]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
Specification
Retina, Muller cells and Horizontal cells
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Jul 03 2013

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