重组Anti-CAPZB抗体[EPR10236] (ab175212)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10236] to CAPZB
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CAPZB抗体[EPR10236]
参阅全部 CAPZB 一抗 -
描述
兔单克隆抗体[EPR10236] to CAPZB -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human CAPZB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P47756 -
阳性对照
- HeLa, 293T, Jurkat and A431 cell lysates; Human kidney and placenta tissues; Jurkat cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR10236 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175212于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
IP |
1/10 - 1/100.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. -
序列相似性
Belongs to the F-actin-capping protein beta subunit family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 832 Human
- Entrez Gene: 12345 Mouse
- Entrez Gene: 298584 Rat
- Omim: 601572 Human
- SwissProt: P47756 Human
- SwissProt: P47757 Mouse
- SwissProt: Q5XI32 Rat
- Unigene: 432760 Human
see all -
别名
- 11 antibody
- Cap protein, actin, beta antibody
- Cap Z antibody
see all
图片
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All lanes : Anti-CAPZB antibody [EPR10236] (ab175212) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZB antibody [EPR10236] (ab175212)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CAPZB with ab175212 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZB antibody [EPR10236] (ab175212)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CAPZB with ab175212 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling CAPZB with ab175212at 1/10 dilution (red) compared toa Rabbit IgG negative control (green).
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab175212.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab175212 被引用在 3 文献中.
- Su X et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis 9:302 (2018). PubMed: 29467473
- Hong F et al. Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90. PLoS One 12:e0169260 (2017). PubMed: 28056051
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152