重组Anti-CAPZA1抗体[EPR11210-4R] (ab175233)
![Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.cn/ps/products/175/ab175233/Images/ab175233-201608-ab175233.jpg "Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11210-4R] to CAPZA1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CAPZA1抗体[EPR11210-4R]
参阅全部 CAPZA1 一抗 -
描述
兔单克隆抗体[EPR11210-4R] to CAPZA1 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Recombinant fragment within Human CAPZA1. The exact sequence is proprietary.
Database link: P52907 -
阳性对照
- HeLa, Jurkat, 293T and HepG2 cell lysates; Human muscle and Human urinary bladder carcinoma tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11210-4R -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175233于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/10 - 1/100.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
|
| 说明 |
|---|
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
靶标
-
功能
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. -
序列相似性
Belongs to the F-actin-capping protein alpha subunit family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 829 Human
- Entrez Gene: 12340 Mouse
- Entrez Gene: 691149 Rat
- Omim: 601580 Human
- SwissProt: P52907 Human
- SwissProt: P47753 Mouse
- SwissProt: B2GUZ5 Rat
- Unigene: 727604 Human
see all -
别名
- Cap Z antibody
- Cappa 1 antibody
- Cappa1 antibody
see all
图片
-
Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)All lanes : Anti-CAPZA1 antibody [EPR11210-4R] (ab175233) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.cn/ps/products/175/ab175233/Images/ab175233-201604-ab175233b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab175233 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.cn/ps/products/175/ab175233/Images/ab175233-201606-ab175233d.jpg)
Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunoprecipitation of CAPZA1 from HepG2 cell lysate using ab175233 at 1/10.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.cn/ps/products/175/ab175233/Images/ab175233-201603-ab175233c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunohistochemical analysis of paraffin embedded Human urinary bladder carcinoma tissue labeling CAPZA1 with ab175233 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.cn/ps/products/175/ab175233/Images/ab175233-5-benefits-of-recombinant-antibodies.png)
Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab175233 尚未被引用在任何文献中。