Anti-Cannabinoid Receptor I抗体(ab40860)
Key features and details
- Goat polyclonal to Cannabinoid Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cannabinoid Receptor I抗体
参阅全部 Cannabinoid Receptor I 一抗 -
描述
山羊多克隆抗体to Cannabinoid Receptor I -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog
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免疫原
Synthetic peptide corresponding to Human Cannabinoid Receptor I aa 320-334 (internal sequence). (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1)
Sequence:HTSEDGKVQVTRPDQ
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阳性对照
- WB: HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
靶标
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功能
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
- SwissProt: P20272 Rat
see all -
别名
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
图片
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Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)All lanes : Anti-Cannabinoid Receptor I antibody (ab40860) at 1 µg/ml
Lane 1 : HeLa lysate in RIPA buffer (non-glycosylated)
Lane 2 : HeLa lysate in RIPA buffer (non-glycosylated) with peptide at 0.5 µg/ml
Lane 3 : HeLa lysate in RIPA buffer (glycosylated)
Lane 4 : HeLa lysate in RIPA buffer (glycosylated) with peptide at 0.5 µg/ml
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (2)
ab40860 被引用在 2 文献中.
- Forte N et al. Orexin-A and endocannabinoids are involved in obesity-associated alteration of hippocampal neurogenesis, plasticity, and episodic memory in mice. Nat Commun 12:6137 (2021). PubMed: 34675233
- Kim J et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . PubMed: 24711795
