Anti-CAMKIV抗体[EP2564Y] (ab75874)
Key features and details
- Rabbit monoclonal [EP2564Y] to CAMKIV
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-CAMKIV抗体[EP2564Y]
参阅全部 CAMKIV 一抗 -
描述
兔单克隆抗体[EP2564Y] to CAMKIV -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat whole cell lysate and Human brain lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2564Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75874于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 52 kDa. |
靶标
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功能
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. -
组织特异性
Expressed in epithelial ovarian cancer tissue. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
细胞定位
Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 814 Human
- Entrez Gene: 12326 Mouse
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P08414 Mouse
- SwissProt: P13234 Rat
- Unigene: 591269 Human
see all -
别名
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
图片
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All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab75874 被引用在 1 文献中.
- Yan X et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . PubMed: 27611779