AB75874

Anti-CAMKIV抗体[EP2564Y]

Anti-CAMKIV antibody [EP2564Y]

RabMAb
了解详情

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(1 Publication)

Rabbit Monoclonal CAMKIV antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

CAMK, CAMK-GR, CAMKIV, CAMK4, Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

1 Images

Unknown

Western blot - Anti-CAMKIV antibody [EP2564Y] (AB75874)

All lanes:

Western blot - Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Human brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CAMKIV antibody [EP2564Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2564Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as CaMKIV (Calcium/Calmodulin-dependent Protein Kinase IV) plays a role in various cellular processes. Mechanically CaMKIV is a serine/threonine protein kinase with a molecular weight of approximately 52 kDa. It depends on calcium ions and calmodulin for activation. The protein is expressed in several tissues including the brain thymus and testis. It is known for its involvement in phosphorylating substrates which then activate transcription factors.

Biological function summary

CaMKIV takes part in the regulation of gene expression and is involved in neural signaling cell cycle control and immune responses. This kinase forms part of a larger signaling complex that facilitates specific cellular events. Its activity affects processes such as long-term potentiation and T-cell maturation indicating its significance in both the nervous and immune systems.

Pathways

CaMKIV integrates into several signaling pathways including the calcium signaling pathway and CREB (cAMP response element-binding protein) pathway. Through these pathways CaMKIV activates CREB via phosphorylation linking it to gene transcription processes essential for memory formation. CaMKIV acts alongside proteins like other CaM kinases such as CaMKI and CaMKII to ensure efficient signal transduction.

CaMKIV's function impacts conditions like neurodegenerative diseases and autoimmune disorders. Dysregulation of CaMKIV may relate to Alzheimer's disease where altered calcium homeostasis contributes to pathogenesis. The protein also connects to autoimmune diseases through its interaction with NFAT (nuclear factor of activated T-cells) which influences immune responses and might lead to disorders when dysregulated.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

See full target information CAMK4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0162784 PubMed27611779

2016

CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation.

Applications

ICC/IF, WB

Species

Rat, Rat

Xiaodong Yan,Juanfang Liu,Zhengxu Ye,Jinghui Huang,Fei He,Wei Xiao,Xueyu Hu,Zhuojing Luo

View all publications

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Anti-CAMKIV抗体[EP2564Y]