AB32678

Anti-CaMKII (phospho T286) 抗体

Name: CaMKII(磷酸化T286)抗体 (ab32678)| Abcam中文官网
Brand: Abcam Limited
SKU: AB32678
Price: 5239 CNY
Availability: InStock
Rating: 5 (28 reviews)

Anti-CaMKII (phospho T286) antibody

(28 Reviews)

(107 Publications)

Anti-CaMKII (phospho T286) antibody (ab32678) is a rabbit polyclonal antibody detecting CaMKII in Western Blot. Suitable for Rat.

- Over 80 publications
- Trusted since 2006

查看别名

Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a

1 Images

Unknown

Western blot - Anti-CaMKII (phospho T286) antibody (AB32678)

Predicted molecular weight : ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.

All lanes:

Western blot - Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution

Lane 1:

Rat brain cortex lysate. at 50 µg

Lane 2:

Rat brain cortex lysate preincubated with lambda-phosphatase. at 50 µg

Predicted band size: 54 kDa

Observed band size: 50 kDa,60 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Rat

应用

applications

免疫原

Synthetic Peptide within Rat Camk2a phospho T286. The exact immunogen used to generate this antibody is proprietary information.

P11275

特异性

This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

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反应性数据

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产品详情

What is this antibody validated in?
Anti-CaMKII (phospho T286) antibody (ab32678) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Rat samples.

What is the molecular weight of CaMKII?
Anti-CaMKII (phospho T286) (ab32678) specifically detects a band for CaMKII (UniProt: Q13554) at a molecular weight of 50kDa.

Trusted by the scientific community
Anti-CaMKII (phospho T286) (ab32678) was first used in a scientific publication in 2006 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-CaMKII (phospho T286) (ab32678) has over 25 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.

Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed : 15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

See full target information Camk2a phospho T286

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文献 (107)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70480 PubMed40070045

2025

Phosphodiesterase 10A Inhibitor Modulates Right Ventricular Outflow Tract Electrophysiological Activities and Calcium Homeostasis via the cGMP/PKG Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Zhi Lin,Yao-Chang Chen,Hsiang-Yu Yang,Wei-Shiang Lin,Yen-Yu Lu

Cardiovascular toxicology 25:34-47 PubMed39521734

2024

Isoprenaline Inhibits Histone Demethylase LSD1 to Induce Cardiac Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Wu,Bo Yang,Yingze Sun,Guanwei Fan,Lina Ma,Ying Ma,Xianjia Xiong,Hui Zhou,Huiping Wang,Ling Zhang,Bing Yang

Nature communications 15:7000 PubMed39143095

2024

Lamin A/C deficiency-mediated ROS elevation contributes to pathogenic phenotypes of dilated cardiomyopathy in iPSC model.

Applications

Species

Human

Hangyuan Qiu,Yaxun Sun,Xiaochen Wang,Tingyu Gong,Jun Su,Jiaxi Shen,Jingjun Zhou,Jiafeng Xia,Hao Wang,Xiangfu Meng,Guosheng Fu,Donghui Zhang,Chenyang Jiang,Ping Liang

Science advances 10:eado0866 PubMed39093964

2024

Modulating amacrine cell-derived dopamine signaling promotes optic nerve regeneration and preserves visual function.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Jingfei Xue,Jiahui Tang,Siting Wu,Zhe Liu,Caiqing Wu,Canying Liu,Yidan Liu,Jicheng Lin,Jiaxu Han,Liyan Liu,Yuze Chen,Jinpeng Yang,Zhidong Li,Ling Zhao,Yantao Wei,Yiqing Li,Yehong Zhuo

International journal of molecular sciences 25: PubMed39062858

2024

Chronic Partial Sleep Deprivation Increased the Incidence of Atrial Fibrillation by Promoting Pulmonary Vein and Atrial Arrhythmogenesis in a Rodent Model.

Applications

Unspecified application

Species

Unspecified reactive species

Shuen-Hsin Liu,Fong-Jhih Lin,Yu-Hsun Kao,Pao-Huan Chen,Yung-Kuo Lin,Yen-Yu Lu,Yao-Chang Chen,Yi-Jen Chen

JACC. Basic to translational science 9:78-96 PubMed38362342

2024

Cardiomyocyte Alpha-1A Adrenergic Receptors Mitigate Postinfarct Remodeling and Mortality by Constraining Necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiandong Zhang,Peyton B Sandroni,Wei Huang,Xiaohua Gao,Leah Oswalt,Melissa A Schroder,SungHo Lee,Yen-Yu I Shih,Hsiao-Ying S Huang,Philip M Swigart,Bat E Myagmar,Paul C Simpson,Joseph S Rossi,Jonathan C Schisler,Brian C Jensen

Cardiovascular research 120:596-611 PubMed38198753

2024

Cardiac monoamine oxidase-A inhibition protects against catecholamine-induced ventricular arrhythmias via enhanced diastolic calcium control.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Shi,Hamza Malik,Rachel M Crawford,Jennifer Streeter,Jinxi Wang,Ran Huo,Jean C Shih,Biyi Chen,Duane Hall,E Dale Abel,Long-Sheng Song,Ethan J Anderson

Communications biology 7:3 PubMed38168628

2024

Activation of M1 cholinergic receptors in mouse somatosensory cortex enhances information processing and detection behaviour.

Applications

Unspecified application

Species

Unspecified reactive species

Wricha Mishra,Ehsan Kheradpezhouh,Ehsan Arabzadeh

Biological research 56:65 PubMed38041203

2023

Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyi Ding,Long Li,Sinuo Wang,Yajun Cao,Minguang Yang,Yaling Dai,Huawei Lin,Jianhong Li,Yulu Liu,Zhifu Wang,Weilin Liu,Jing Tao

International journal of molecular sciences 24: PubMed38003332

2023

Ketogenic Diet Regulates Cardiac Remodeling and Calcium Homeostasis in Diabetic Rat Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-I Lee,Nguyen Ngoc Trang,Ting-Wei Lee,Satoshi Higa,Yu-Hsun Kao,Yao-Chang Chen,Yi-Jen Chen

View all publications

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Anti-CaMKII (phospho T286) 抗体

Anti-CaMKII (phospho T286) antibody

Western blot - Anti-CaMKII (phospho T286) antibody (AB32678)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性