重组Anti-Calsequestrin 2抗体[EPR4228] (ab108381)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121020-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4228] to Calsequestrin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Calsequestrin 2抗体[EPR4228]
参阅全部 Calsequestrin 2 一抗 -
描述
兔单克隆抗体[EPR4228] to Calsequestrin 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human heart and Human skeletal muscle lysates, Human cardiac muscles and Human skeletal muscles tissues
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4228 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108381于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. -
疾病相关
Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. -
序列相似性
Belongs to the calsequestrin family. -
细胞定位
Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 845 Human
- Omim: 114251 Human
- SwissProt: O14958 Human
- Unigene: 57975 Human
-
别名
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-286117-anti-calsequestrin-2-antibody-epr4228-western-blot.jpg)
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/10000 dilution (purified)
Lane 1 : Human fetal heart lysates
Lane 2 : Human skeletal muscle lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-286439-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1:250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
-
![Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121022-anti-calsequestrin-2-antibody-epr4228-western-blot.jpg)
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/1000 dilution (unpurified)
Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121021-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121019-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Unpurified ab108381 showing negative staining in Normal brain tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121018-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Unpurified ab108381 showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121017-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Unpurified ab108381 showing negative staining in Normal kidney tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121016-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Unpurified ab108381 showing negative staining in Normal colon tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-121015-anti-calsequestrin-2-antibody-epr4228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)Unpurified ab108381 showing negative staining in Normal tonsil tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
![Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)](https://www.abcam.cn/ps/products/108/ab108381/Images/ab108381-11-benefits-of-recombinant-antibodies.png)
Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab108381 尚未被引用在任何文献中。