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重组RabMAb

重组Anti-Calretinin抗体[EPR1799(2)] (ab133316)

Reviews (2)Q&A (1)
Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
  • Anti-Calretinin antibody [EPR1799(2)] (ab133316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1799(2)] to Calretinin
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Calretinin抗体[EPR1799(2)]
    参阅全部 Calretinin 一抗

  • 描述

    兔单克隆抗体[EPR1799(2)] to Calretinin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt or IHC-P

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Fetal brain, SH-SY5Y, 293T, fetal kidney, and U87-MG lysates

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR1799(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133316于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
应用说明
Is unsuitable for Flow Cyt or IHC-P.

靶标

图片

  • Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
    Western blot - Anti-Calretinin antibody [EPR1799(2)] (ab133316)
    All lanes : Anti-Calretinin antibody [EPR1799(2)] (ab133316) at 1/1000 dilution

    Lane 1 : Fetal brain lysates
    Lane 2 : SH-SY5Y lysates
    Lane 3 : 293T lysates
    Lane 4 : Fetal kidney lysates
    Lane 5 : U87-MG lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • Anti-Calretinin antibody [EPR1799(2)] (ab133316)
    Anti-Calretinin antibody [EPR1799(2)] (ab133316)

文献 (0)

发表研究结果有使用 ab133316？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab133316 尚未被引用在任何文献中。

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Calretinin antibody [EPR1799(2)]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Neuron)
Specification
Neuron
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 31 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Calretinin [EPR1799(2)] antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

提交于 Jul 18 2014

Question

I would ask an information about your primary antibodies: anti-Calretinin EPR 1799(2) cod. ab 133316 and anti-Calretinin EP 1798 cod. ab 92341. The SH-SY5Y cells, used as positive controls in Western Blotting and immuonfluorescence, are undifferentiated or differentiated cells?

Read More
Answer


The SH-SY5Y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH. SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA.

Read More

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