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Anti-Calretinin抗体(ab702)

Reviews (4)Q&A (3)References (59)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

    Key features and details

    • Rabbit polyclonal to Calretinin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Calretinin antibody [EP1798] (ab92341)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Calretinin抗体
      参阅全部 Calretinin 一抗

    • 描述

      兔多克隆抗体to Calretinin

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Chicken, Cat, Dog, Xenopus laevis

    • 免疫原

      Full length protein corresponding to Calretinin.

    • 常规说明

      Contains a proprietary preservative that is not sodium azide or thimerosal.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituents: PBS, Carrier protein

      Van Gogh Yellow Diluent
    • Concentration information loading...

    • 纯度

      Whole antiserum

    • 克隆

      多克隆

    • 骨髓瘤

      unknown

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab702于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
    说明
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

    靶标

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

      Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

    文献 (59)

    发表研究结果有使用 ab702？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab702 被引用在 59 文献中.

    • Nieuwenhuis B  et al. Improving adeno-associated viral (AAV) vector-mediated transgene expression in retinal ganglion cells: comparison of five promoters. Gene Ther N/A:N/A (2023). PubMed: 36635457
    • Tan H  et al. Morphological and distributional properties of SMI-32 immunoreactive ganglion cells in the rat retina. J Comp Neurol 530:1276-1287 (2022). PubMed: 34802150
    • Guo Q  et al. Calreticulin Identified as One of the Androgen Response Genes That Trigger Full Regeneration of the Only Capable Mammalian Organ, the Deer Antler. Front Cell Dev Biol 10:862841 (2022). PubMed: 35769266
    • Lai HM  et al. Antibody stabilization for thermally accelerated deep immunostaining. Nat Methods 19:1137-1146 (2022). PubMed: 36050489
    • Kawai M  et al. Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor function. Cell Rep 37:110019 (2021). PubMed: 34818559
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calretinin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain(Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain(Hippocampus)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. J. Y. Seo

    Verified customer

    提交于 Apr 03 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (lung)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    lung
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. EUN JEUNG KIM

    Verified customer

    提交于 Jan 12 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Fukomys anselli Tissue sections (Retina)
    Permeabilization
    Yes - Triton X-100 in Blocking Solution
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Davidson's Fixative
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 31 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
    Sample
    Mouse Tissue sections (frozen brain section)
    Specification
    frozen brain section
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Bettina Hoerz

    Verified customer

    提交于 Jan 06 2014

    Question

    I would like to know the expiration date of the antibody ab702, lot number 877133.

    Read More
    Answer



    We do not have specific use by dates for our products.

    If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

    Please review and follow carefully the storage instructions on the individual datasheets.

    For more tips on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    We suggest the following approximate use by dates for antibodies depending on which temperature they are recommended to be stored at:

    room temperature: 1 week

    + 4°C: 1 year

    - 20°C: 5 years

    - 80°C: 10 years

    Other product types are also guaranteed for 1 year. However, specific storage instructions are provided on datasheets and protocol booklets. Sometimes kit components will have a shorter shelf life once reconstituted or prepared and details are provided in protocol booklets for kits.

    Read More

    Question

    Estimado:
    Estuve conversando con el cliente, las publicaciones que necesita es relacionado con las referencias en donde se haya utilizado el mesotelioma como control positivo para Calretinina (ab702).
    Espero que me puedas ayudar con esto.
    Saludos cordiales

    Read More
    Answer

    Gracias por tu respuesta.

    Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

    Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas técnicas encuentre exactamente lo que está buscando. El enlace a las distintas alternativas es el siguiente:

    https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=Calretinin

    Espero que consiga la información que necesita. Para cualquier otra información, no dudes en volvernos a contactar.

    Read More

    Question

    Buenas tardes, podrian por favor enviarme los articulos referenciados en la ficha tecnica de ab702? No consigo acceder a ellos.
    Muchas gracias.

    Read More
    Answer

    Gracias por contactarnos.


    Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuación le llevan directamente al artículo:

    http://www.jneurosci.org/content/31/25/9368.long

    http://dev.biologists.org/content/137/13/2107.long

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517219/?tool=pubmed

    http://cercor.oxfordjournals.org/content/18/7/1604.long

    http://toxsci.oxfordjournals.org/content/89/1/314.long

    El resto de artículos no son de libre acceso, es necesario suscribirse a la revista para poder acceder a sus publicaciones. Si no son miembros de la revista, no podrán acceder a la misma online, y me temo que solo podrán consultar los abstracts.

    Espero que esta información sea de utilidad, en caso contrario, no dudes en volverme a contactar.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com