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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins

Anti-Calretinin抗体(ab702)

  • Datasheet
  • SDS
Reviews (4)Q&A (3)References (50)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

    Key features and details

    • Rabbit polyclonal to Calretinin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Calretinin antibody [EP1798] (ab92341)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Calretinin抗体
      参阅全部 Calretinin 一抗
    • 描述

      兔多克隆抗体to Calretinin
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
      预测可用于: Chicken, Cat, Dog, Xenopus laevis
    • 免疫原

      Full length protein corresponding to Calretinin.

    • 常规说明

      Contains a proprietary preservative that is not sodium azide or thimerosal.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituents: PBS, Carrier protein

      Van Gogh Yellow Diluent
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 骨髓瘤

      unknown
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Calcium Signaling
      • Calcium Binding Proteins
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Calretinin protein (ab100941)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab702于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

    说明
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

    靶标

    • 功能

      Calretinin is a calcium-binding protein which is abundant in auditory neurons.
    • 组织特异性

      Brain.
    • 序列相似性

      Belongs to the calbindin family.
      Contains 6 EF-hand domains.
    • Target information above from: UniProt accession P22676 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 396255 Chicken
      • Entrez Gene: 794 Human
      • Omim: 114051 Human
      • SwissProt: P07090 Chicken
      • SwissProt: P22676 Human
      • Unigene: 106857 Human
      • 别名

        • 29 kDa calbindin antibody
        • CAB 29 antibody
        • CAB29 antibody
        • CAL 2 antibody
        • CAL2 antibody
        • CALB 2 antibody
        • CALB2 antibody
        • CALB2_HUMAN antibody
        • Calbindin 2 29kDa antibody
        • Calbindin 2 antibody
        • Calbindin D29K antibody
        • Calbindin2 antibody
        • Calretinin antibody
        • CR antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

        Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (50)

      发表研究结果有使用 ab702?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab702 被引用在 50 文献中.

      • Ribeiro J  et al. Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors. Cell Rep 35:109022 (2021). PubMed: 33882303
      • Khatib TZ  et al. Receptor-ligand supplementation via a self-cleaving 2A peptide-based gene therapy promotes CNS axonal transport with functional recovery. Sci Adv 7:N/A (2021). PubMed: 33789891
      • Torres-Gomez S  et al. Changes in the Proportion of Inhibitory Interneuron Types from Sensory to Executive Areas of the Primate Neocortex: Implications for the Origins of Working Memory Representations. Cereb Cortex 30:4544-4562 (2020). PubMed: 32227119
      • Makeyeva Y  et al. Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa. J Histochem Cytochem 68:491-513 (2020). PubMed: 32603211
      • Prahst C  et al. Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy. Elife 9:N/A (2020). PubMed: 32073398
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calretinin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Brain(Hippocampus))
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.2% Triton X
      Specification
      Brain(Hippocampus)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mr. J. Y. Seo

      Verified customer

      提交于 Apr 03 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Dog Tissue sections (lung)
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Specification
      lung
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MRS. EUN JEUNG KIM

      Verified customer

      提交于 Jan 12 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Fukomys anselli Tissue sections (Retina)
      Permeabilization
      Yes - Triton X-100 in Blocking Solution
      Specification
      Retina
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
      Fixative
      Davidson's Fixative
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 31 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
      Sample
      Mouse Tissue sections (frozen brain section)
      Specification
      frozen brain section
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MRS. Bettina Hoerz

      Verified customer

      提交于 Jan 06 2014

      Question

      I would like to know the expiration date of the antibody ab702, lot number 877133.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 13 2013

      Answer



      We do not have specific use by dates for our products.

      If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

      Please review and follow carefully the storage instructions on the individual datasheets.

      For more tips on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols).

      We suggest the following approximate use by dates for antibodies depending on which temperature they are recommended to be stored at:

      room temperature: 1 week

      + 4°C: 1 year

      - 20°C: 5 years

      - 80°C: 10 years

      Other product types are also guaranteed for 1 year. However, specific storage instructions are provided on datasheets and protocol booklets. Sometimes kit components will have a shorter shelf life once reconstituted or prepared and details are provided in protocol booklets for kits.

      Read More

      Abcam Scientific Support

      回复于 Jun 13 2013

      Question

      Estimado:
      Estuve conversando con el cliente, las publicaciones que necesita es relacionado con las referencias en donde se haya utilizado el mesotelioma como control positivo para Calretinina (ab702).
      Espero que me puedas ayudar con esto.
      Saludos cordiales

      Read More

      Abcam community

      Verified customer

      Asked on Apr 10 2012

      Answer

      Gracias por tu respuesta.

      Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

      Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas técnicas encuentre exactamente lo que está buscando. El enlace a las distintas alternativas es el siguiente:

      https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=Calretinin

      Espero que consiga la información que necesita. Para cualquier otra información, no dudes en volvernos a contactar.

      Read More

      Abcam Scientific Support

      回复于 Apr 10 2012

      Question

      Buenas tardes, podrian por favor enviarme los articulos referenciados en la ficha tecnica de ab702? No consigo acceder a ellos.
      Muchas gracias.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 04 2012

      Answer

      Gracias por contactarnos.


      Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuación le llevan directamente al artículo:

      http://www.jneurosci.org/content/31/25/9368.long

      http://dev.biologists.org/content/137/13/2107.long

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517219/?tool=pubmed

      http://cercor.oxfordjournals.org/content/18/7/1604.long

      http://toxsci.oxfordjournals.org/content/89/1/314.long

      El resto de artículos no son de libre acceso, es necesario suscribirse a la revista para poder acceder a sus publicaciones. Si no son miembros de la revista, no podrán acceder a la misma online, y me temo que solo podrán consultar los abstracts.

      Espero que esta información sea de utilidad, en caso contrario, no dudes en volverme a contactar.

      Read More

      Abcam Scientific Support

      回复于 Apr 04 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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