Anti-Calprotectin (S100A8 + S100A9)抗体[5.5] (ab20222)
Key features and details
- Mouse monoclonal [5.5] to Calprotectin (S100A8 + S100A9)
- Suitable for: IHC-Fr, IP, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Calprotectin (S100A8 + S100A9)抗体[5.5]
参阅全部 Calprotectin (S100A8 + S100A9) 一抗 -
描述
小鼠单克隆抗体[5.5] to Calprotectin (S100A8 + S100A9) -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human S100A9+S100A8. acute monocytic leukaemia cells (Human).
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阳性对照
- Neutrophil lysate (particularly neutrophils from spleen)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
5.5 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
Predicted molecular weight MRP8 10.8 kDa, MRP14 13 kDa. |
| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight MRP8 10.8 kDa, MRP14 13 kDa. |
靶标
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细胞定位
S100A9: Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. S100A8: Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway. -
数据库链接
- Entrez Gene: 6279 Human
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P05109 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
- Unigene: 416073 Human
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab20222 被引用在 4 文献中.
- Wen H et al. Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma. Nat Med 24:154-164 (2018). WB . PubMed: 29291352
- Wang Y et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). WB, IHC ; Mouse . PubMed: 24691441
- Hessian PA & Fisher L The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure. Eur J Biochem 268:353-63 (2001). PubMed: 11168370
- Hogg N et al. Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium. Eur J Immunol 19:1053-61 (1989). PubMed: 2666142