Anti-Calponin 2抗体(ab129331)
Key features and details
- Goat polyclonal to Calponin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Calponin 2抗体 -
描述
山羊多克隆抗体to Calponin 2 -
宿主
Goat -
特异性
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Calponin 2 aa 292-306 (internal sequence). Near the C terminus of Human Calponin 2 (NP_004359.1).
Sequence:C-DPGEVPEYPPYYQEE
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阳性对照
- Human Placenta tissue; HepG2 lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
组织特异性
Heart and smooth muscle. -
序列相似性
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1265 Human
- Omim: 602373 Human
- SwissProt: Q99439 Human
- Unigene: 651512 Human
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别名
- Calponin 2 antibody
- Calponin H2 antibody
- Calponin H2, smooth muscle antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (ab129331)
ab129331, at 2.5 µg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
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Anti-Calponin 2 antibody (ab129331) at 0.03 µg/ml + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab129331 尚未被引用在任何文献中。