重组Anti-Calponin 1抗体[EP798Y] (ab46794)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP798Y] to Calponin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
Related conjugates and formulations
概述
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产品名称
Anti-Calponin 1抗体[EP798Y]
参阅全部 Calponin 1 一抗 -
描述
兔单克隆抗体[EP798Y] to Calponin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Sheep -
免疫原
Synthetic peptide within Human Calponin 1 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P51911 -
阳性对照
- ICC/IF: C2C12 and HeLa cells; Pig and mouse aortic smooth muscle cells. IHC-P: Rat lung tissue; Mouse cardiac muscle tissue; Human lung carcinoma, kidney, lung, tonsil, uterus, smooth muscle and skeletal muscle tissues. WB: Human bladder lysates, Pig heart lysates, Mouse bladder lysates and Rat bladder lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 1.73 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP798Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46794于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (9) |
1/5000. Detects a band of approximately 34 kDa.
For unpurified use at 1/20,000. |
IHC-P | (3) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. |
ICC/IF | (4) |
1/500.
For unpurified use at 1/100 - 1/250. |
说明 |
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WB
1/5000. Detects a band of approximately 34 kDa. For unpurified use at 1/20,000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
靶标
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功能
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
组织特异性
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
序列相似性
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1264 Human
- Entrez Gene: 12797 Mouse
- Entrez Gene: 396911 Pig
- Entrez Gene: 65204 Rat
- Entrez Gene: 443517 Sheep
- Omim: 600806 Human
- SwissProt: P51911 Human
- SwissProt: Q08091 Mouse
see all -
别名
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Calponin 1 with purified ab46794 at 1/500 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/50000 dilution (purified)
Lane 1 : Human bladder lysates
Lane 2 : Mouse bladder lysates
Lane 3 : Rat bladder lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilutionBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Calponin 1 with purified ab46794 at a 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
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Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)Torrado M et al. Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart. PLoS One 6:e26392 (2011). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794)
Lanes 1-2 : Non-Failing pig LV-myocardium tissue lysate.
Lanes 3-4 : Failing pig LV-myocardium tissue lysate injected with DOX.Ab46794 was used to stain Calponin 1 in Neonatal piglets injected with Dox (Failing LV myocardium) and PBS (Non-Failing LV myocardium). At the protein level a more than twofold increase in Calponin 1 was observed in Dox-injected animals compared to controls (PBS).
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Paraformadehyde-fixed, 0.25% Triton X-100 permeabilized mouse thoracic aortic smooth muscle cells labeling Calponin 1 using ab46794 at 1/100 dilution in ICC/IF, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150077) at 1/400 dilution.
1.5% BSA used used as blocking agent for 30 minutes at 25°C. Incubated with primary antibody for 24 hours at 4°C.
VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells. After fixation, VSMCs were treated with 0.25% Triton X-100 for 20 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
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Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/5000 dilution (purified) + Pig heart lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody.
PBS instead of the primary antibody was used as the negative control (inset).
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Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)Image kindly supplied by Mrs Jordan Carbary through Abreview
Unpurified ab46794 staining Calponin 1 in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence.
The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)Herington et al PLoS One. 2015 Nov 24;10(11):e0143243. doi: 10.1371/journal.pone.0143243. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Representative photomicrograph of mouse UT-myo cells (Left panel) and murine uterine myometrium (Right panel) stained with smooth muscle cell markers, alpha-SMA (red) and ab46794 (green) and DAPI (blue). UT-myo cells and whole-mount uterine tissue were collected from day 19 of mouse pregnancy. The placenta and embryo were removed from whole-mount tissue sections.
For full details please see paper.
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Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified) + Human bladder lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ICC/IF image of unpurified ab46794 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
Cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemical staining of paraffin-embedded human smooth muscle using unpurified ab46794 at 1/100 dilution
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All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified)
All lanes : Whole tissue lysate prepared from bovine lymph vessels
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal lung vessel tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal kidney vessels tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal tonsil vessel tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal uterus tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing negative staining in skeletal muscle tissue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (234)
ab46794 被引用在 234 文献中.
- Liu S et al. Immunomodulatory hybrid micro-nanofiber scaffolds enhance vascular regeneration. Bioact Mater 21:464-482 (2023). PubMed: 36185748
- Yu Q et al. Sox9 mediates autophagy-dependent vascular smooth muscle cell phenotypic modulation and transplant arteriosclerosis. iScience 25:105161 (2022). PubMed: 36204267
- Szczepanski HE et al. NR4A1 modulates intestinal smooth muscle cell phenotype and dampens inflammation-associated intestinal remodeling. FASEB J 36:e22609 (2022). PubMed: 36250380
- Tai S et al. Metformin suppresses vascular smooth muscle cell senescence by promoting autophagic flux. J Adv Res 41:205-218 (2022). PubMed: 36328749
- Lee S et al. High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates. Sci Rep 12:317 (2022). PubMed: 35013350