Anti-Calpain 5抗体(ab28280)
Key features and details
- Rabbit polyclonal to Calpain 5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Calpain 5抗体
参阅全部 Calpain 5 一抗 -
描述
兔多克隆抗体to Calpain 5 -
宿主
Rabbit -
特异性
Does not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.). -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Calpain 5 aa 1-30 (N terminal).
(Peptide available asab41310) -
常规说明
Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been peptide-affinity purified. -
Primary antibody说明
ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28280于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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组织特异性
Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer. -
疾病相关
Vitreoretinopathy, neovascular inflammatory -
序列相似性
Belongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain. - Information by UniProt
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数据库链接
- Entrez Gene: 726 Human
- Omim: 602537 Human
- SwissProt: O15484 Human
- Unigene: 248153 Human
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别名
- ADNIV antibody
- Calpain htra 3 antibody
- Calpain htra-3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Calpain 5 antibody (ab28280)ICC/IF image of ab28280 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab28280 被引用在 3 文献中.
- Nakashima N et al. Olfactory receptor neurons express olfactory marker protein but not calpain 5 from the same genomic locus. Mol Brain 12:54 (2019). PubMed: 31164142
- Schaefer KA et al. Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses. Invest Ophthalmol Vis Sci 57:2509-21 (2016). PubMed: 27152965
- Singh R et al. Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies. J Biol Chem 289:19383-94 (2014). WB ; Human, Rat . PubMed: 24838245