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AB28258

Anti-Calpain 1 抗体

Anti-Calpain 1 antibody

5

(6 Reviews)

|

(27 Publications)

Rabbit Polyclonal Calpain 1 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN1.

查看别名

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

1 Images
Western blot - Anti-Calpain 1 antibody (AB28258)
  • WB

Unknown

Western blot - Anti-Calpain 1 antibody (AB28258)

All lanes:

Western blot - Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution

All lanes:

Calpain 1 at 10 µg

Predicted band size: 82 kDa

Observed band size: 80 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

应用

WB

applications

免疫原

Synthetic Peptide within Human CAPN1. The exact immunogen used to generate this antibody is proprietary information.

P07384

特异性

ab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.</p>" } } }
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您是否考虑以下替代产品？

AB108400

Anti-Calpain 1 antibody [EPR3319]

4
1 Reviews
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为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
The antibody has been peptide-affinity purified.
存储溶液
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.
Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).
See full target information CAPN1
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文献 (27)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8177 PubMed38071198

2023

Harnessing PROTAC technology to combat stress hormone receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mahshid Gazorpak,Karina M Hugentobler,Dominique Paul,Pierre-Luc Germain,Miriam Kretschmer,Iryna Ivanova,Selina Frei,Kei Mathis,Remo Rudolf,Sergio Mompart Barrenechea,Vincent Fischer,Xiaohan Xue,Aleksandra L Ptaszek,Julian Holzinger,Mattia Privitera,Andreas Hierlemann,Onno C Meijer,Robert Konrat,Erick M Carreira,Johannes Bohacek,Katharina Gapp

Experimental and therapeutic medicine 25:196 PubMed37090078

2023

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yan Wang,Jia-Xin Shi,Meng-Hua Chen,Xiang-Zhen Zhuge,Chun-Guang Dai,Lu Xie

iScience 24:103056 PubMed34755080

2021

Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kang Tang,Yu-Hsien Lin,Joe-Air Jiang,Yun-Heng Lu,Chih-Hsuan Tsai,Yu-Chun Lin,Yun-Ru Chen,Carol-P Wu,Yueh-Lung Wu

International journal of molecular sciences 22: PubMed33919234

2021

Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Sukocheva,Dong Gui Hu,Robyn Meech,Anupam Bishayee

Biomedicines 9: PubMed33802221

2021

Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Wei Lu,Tzu-Yu Lin,Tai-Long Pan,Pei-Wen Wang,Kuan-Ming Chiu,Ming-Yi Lee,Su-Jane Wang

Doklady. Biochemistry and biophysics 495:338-341 PubMed33368047

2020

Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Yu V Gritsyna,A D Ulanova,S S Popova,A G Bobylev,V K Zhalimov,T L Nemirovskaya,B S Shenkman,I M Vikhlyantsev

Scientific reports 10:15185 PubMed32938992

2020

Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Popova,Anna Ulanova,Yulia Gritsyna,Nikolay Salmov,Vadim Rogachevsky,Gulnara Mikhailova,Alexander Bobylev,Liya Bobyleva,Yana Yutskevich,Oleg Morenkov,Nadezda Zakharova,Ivan Vikhlyantsev

Journal of agricultural and food chemistry 67:11718-11727 PubMed31518118

2019

Expression of Pork Plectin during Postmortem Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaona Tian,Yingying Wang,Xiaoquan Fan,Yingwu Shi,Wangang Zhang,Qin Hou,Rui Liu,Guanghong Zhou

Physiological reports 7:e14218 PubMed31456346

2019

Altered oxidative stress and antioxidant defence in skeletal muscle during the first year following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mladen Savikj,Emil Kostovski,Leonidas S Lundell,Per O Iversen,Julie Massart,Ulrika Widegren

Journal of the American Society of Nephrology : JA 30:1910-1924 PubMed31416818

2019

TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility.

Applications

Unspecified application

Species

Unspecified reactive species

Louise K Farmer,Ruth Rollason,Daniel J Whitcomb,Lan Ni,Alexander Goodliff,Abigail C Lay,Lutz Birnbaumer,Kate J Heesom,Shang-Zhong Xu,Moin A Saleem,Gavin I Welsh
View all publications
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