AB28258

Anti-Calpain 1 抗体

Name: Calpain 1抗体 (ab28258)| Abcam中文官网
Brand: Abcam Limited
SKU: AB28258
Price: 4923 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-Calpain 1 antibody

(6 Reviews)

(32 Publications)

Rabbit Polyclonal Calpain 1 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN1.

查看别名

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

1 Images

Unknown

Western blot - Anti-Calpain 1 antibody (AB28258)

All lanes:

Western blot - Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution

All lanes:

Calpain 1 at 10 µg

Predicted band size: 82 kDa

Observed band size: 80 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

应用

applications

免疫原

Synthetic Peptide within Human CAPN1. The exact immunogen used to generate this antibody is proprietary information.

P07384

特异性

ab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.).

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.</p>" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

The antibody has been peptide-affinity purified.

存储溶液

Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.

Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).

See full target information CAPN1

style

left-column

文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed40430436

2025

Epigallocatechin Gallate in Ameliorates Skin Aging by Reducing Mitochondrial ROS Production.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Ho Park,Eun Young Jeong,Ye Hyang Kim,So Yoon Cha,Ha Yeon Kim,Yeon Kyung Nam,Jin Seong Park,So Yeon Kim,Yoo Jin Lee,Jee Hee Yoon,Byeonghyeon So,Duyeol Kim,Minseon Kim,Youngjoo Byun,Yun Haeng Lee,Song Seok Shin,Joon Tae Park

Nature communications 14:8177 PubMed38071198

2023

Harnessing PROTAC technology to combat stress hormone receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mahshid Gazorpak,Karina M Hugentobler,Dominique Paul,Pierre-Luc Germain,Miriam Kretschmer,Iryna Ivanova,Selina Frei,Kei Mathis,Remo Rudolf,Sergio Mompart Barrenechea,Vincent Fischer,Xiaohan Xue,Aleksandra L Ptaszek,Julian Holzinger,Mattia Privitera,Andreas Hierlemann,Onno C Meijer,Robert Konrat,Erick M Carreira,Johannes Bohacek,Katharina Gapp

Experimental and therapeutic medicine 25:196 PubMed37090078

2023

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yan Wang,Jia-Xin Shi,Meng-Hua Chen,Xiang-Zhen Zhuge,Chun-Guang Dai,Lu Xie

International journal of molecular sciences 23: PubMed36499770

2022

Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models.

Applications

Unspecified application

Species

Unspecified reactive species

John A Miller,Domenica E Drouet,Leonid M Yermakov,Mahmoud S Elbasiouny,Fatima Z Bensabeur,Michael Bottomley,Keiichiro Susuki

iScience 24:103056 PubMed34755080

2021

Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kang Tang,Yu-Hsien Lin,Joe-Air Jiang,Yun-Heng Lu,Chih-Hsuan Tsai,Yu-Chun Lin,Yun-Ru Chen,Carol-P Wu,Yueh-Lung Wu

International journal of molecular sciences 22: PubMed33919234

2021

Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Sukocheva,Dong Gui Hu,Robyn Meech,Anupam Bishayee

Biomedicines 9: PubMed33802221

2021

Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Wei Lu,Tzu-Yu Lin,Tai-Long Pan,Pei-Wen Wang,Kuan-Ming Chiu,Ming-Yi Lee,Su-Jane Wang

Doklady. Biochemistry and biophysics 495:338-341 PubMed33368047

2020

Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Yu V Gritsyna,A D Ulanova,S S Popova,A G Bobylev,V K Zhalimov,T L Nemirovskaya,B S Shenkman,I M Vikhlyantsev

Biomedicine & pharmacotherapy = Biomedecine & phar 131:110788 PubMed33152946

2020

Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinar Rostami,Maria Jäntti,Hengjing Cui,Maiju K Rinne,Jyrki P Kukkonen,Anna Falk,Anna Erlandsson,Timo Myöhänen

Scientific reports 10:15185 PubMed32938992

2020

Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Popova,Anna Ulanova,Yulia Gritsyna,Nikolay Salmov,Vadim Rogachevsky,Gulnara Mikhailova,Alexander Bobylev,Liya Bobyleva,Yana Yutskevich,Oleg Morenkov,Nadezda Zakharova,Ivan Vikhlyantsev

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calpain 1 抗体

Anti-Calpain 1 antibody

Western blot - Anti-Calpain 1 antibody (AB28258)