Anti-Calpain 1抗体(ab28257)
Key features and details
- Rabbit polyclonal to Calpain 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calpain 1抗体
参阅全部 Calpain 1 一抗 -
描述
兔多克隆抗体to Calpain 1 -
宿主
Rabbit -
特异性
ab28257 recognizes the amino terminal end of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also, it does not cross react with the other calpain family members (M Calpain, Calpain 94, ncl2, ncl3, etc.). -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Calpain 1 aa 1-30.
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阳性对照
- Human condrosarcoma cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been peptide-affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28257 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28257 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
细胞定位
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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数据库链接
- Entrez Gene: 823 Human
- Omim: 114220 Human
- SwissProt: P07384 Human
- Unigene: 502842 Human
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别名
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (ab28257)ICC/IF image of ab28257 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28257, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Calpain 1 antibody (ab28257)All lanes : Anti-Calpain 1 antibody (ab28257) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Additional bands at: 27 kDa, 42 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab28257 被引用在 11 文献中.
- Bondada V et al. The C2 domain of calpain 5 contributes to enzyme activation and membrane localization. Biochim Biophys Acta Mol Cell Res 1868:119019 (2021). PubMed: 33811937
- Cai Z et al. Use of a Mouse Model and Human Umbilical Vein Endothelial Cells to Investigate the Effect of Arsenic Exposure on Vascular Endothelial Function and the Associated Role of Calpains. Environ Health Perspect 127:77003 (2019). PubMed: 31274337
- Scott IC et al. Interleukin-33 is activated by allergen- and necrosis-associated proteolytic activities to regulate its alarmin activity during epithelial damage. Sci Rep 8:3363 (2018). PubMed: 29463838
- Tan WJ et al. Calpain 1 regulates TGF-ß1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway. Am J Transl Res 9:1402-1409 (2017). WB ; Human . PubMed: 28386365
- Marcassa E et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open 6:551-562 (2017). PubMed: 28302665