Anti-Calnexin - ER膜marker抗体- ER Marker (ab13504)
Key features and details
- Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Calnexin - ER膜marker抗体- ER Marker
参阅全部 Calnexin 一抗 -
描述
兔多克隆抗体to Calnexin - ER膜marker - ER Marker -
宿主
Rabbit -
特异性
Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis. -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Dog Calnexin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P24643 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| ICC/IF | (5) | |
| WB | (3) |
| 说明 |
|---|
ICC: 1/200.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 18621691).
IP: 1/100.
WB: 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- SwissProt: P35565 Rat
- Unigene: 567968 Human
see all -
别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin’s Fixative Solution. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.
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Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution
All lanes : Mixture of rat tissue lysates
Predicted band size: 90 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody: FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab13504 被引用在 36 文献中.
- Murakami A et al. Cell-autonomous control of intracellular temperature by unsaturation of phospholipid acyl chains. Cell Rep 38:110487 (2022). PubMed: 35294880
- Esfahanian N et al. Comprehensive Analysis of Proteasomal Complexes in Mouse Brain Regions Detects ENO2 as a Potential Partner of the Proteasome in the Striatum. Cell Mol Neurobiol 42:2305-2319 (2022). PubMed: 34037901
- Liu YY et al. Fatty Acid Synthase Is Involved in Classical Swine Fever Virus Replication by Interaction with NS4B. J Virol 95:e0078121 (2021). PubMed: 34132567
- Zhang L et al. S100A8 promotes chemoresistance via augmenting autophagy in B-cell lymphoma cells. Oncol Rep 45:151-158 (2021). PubMed: 33169801
- Li Y et al. MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme. Nat Commun 11:4837 (2020). PubMed: 32973183