重组Anti-Calnexin抗体[EPR21240] - ER膜Marker (ab241154)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21240] to Calnexin - ER Membrane Marker
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calnexin抗体[EPR21240] - ER膜Marker
参阅全部 Calnexin 一抗 -
描述
兔单克隆抗体[EPR21240] to Calnexin - ER膜Marker -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, NIH3T3 and MEF1 cells
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常规说明
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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克隆
单克隆 -
克隆编号
EPR21240 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab241154于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
Abcam recommends a 5% milk block for this product. |
说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Abcam recommends a 5% milk block for this product. |
靶标
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功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
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All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CANX KO whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lane 4 : MEF1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?ab241154 was shown to specifically react with CANX (Calnexin) in wild type HAP1 cells. No band was observed when CANX (calnexin) knockout samples were used. Wild-type and CANX (calnexin) knockout samples were subjected to SDS-PAGE. ab241154 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/ml dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab241154 被引用在 1 文献中.
- Feng D et al. BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP via Delivery of miR-877-3p. Oxid Med Cell Longev 2022:8825784 (2022). PubMed: 35281474