Anti-Calnexin抗体[CANX/1543] (ab238078)
![Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)](https://www.abcam.cn/ps/products/238/ab238078/Images/ab238078-454789-anti-calnexin-antibody-canx1543-western-blot-wildtype-hek293t-canx-knockout-hek293t-u2-os-mcf7.jpg "Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)")
Key features and details
- Mouse monoclonal [CANX/1543] to Calnexin
- Suitable for: Protein Array, IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Calnexin抗体[CANX/1543]
参阅全部 Calnexin 一抗 -
描述
小鼠单克隆抗体[CANX/1543] to Calnexin -
宿主
Mouse -
经测试应用
适用于: Protein Array, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Calnexin aa 1-300. The exact sequence is proprietary.
Database link: P27824 -
阳性对照
- IHC-P: Human renal cell carcinma tissue. WB: HEK-293T, U-2 OS, PANC1 and MCF7 cell lysates; Human kidney lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from Bioreactor Concentrate by Protein A/G. -
克隆
单克隆 -
克隆编号
CANX/1543 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab238078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
|
| 说明 |
|---|
|
Protein Array
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa. |
靶标
-
功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 821 Human
- Omim: 114217 Human
- SwissProt: P27824 Human
- Unigene: 567968 Human
-
别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
-
Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)All lanes : Anti-Calnexin antibody [CANX/1543] (ab238078) at 1 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CANX knockout HEK-293T cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab238078 observed at 80 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab238078 was shown to react with Calnexin in wild-type HEK-293T cells in Western blot with loss of signal observed in CANX knockout cell line ab255368 (CANX knockout cell lysate ab263805). Wild-type HEK-293T and CANX knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab238078 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [CANX/1543] (ab238078)](https://www.abcam.cn/ps/products/238/ab238078/Images/ab238078-315574-anti-calnexin-antibody-canx-1543-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [CANX/1543] (ab238078)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Calnexin using ab238078 at 2 µg/ml in immunohistochemical analysis.
-
![Protein Array - Anti-Calnexin antibody [CANX/1543] (ab238078)](https://www.abcam.cn/ps/products/238/ab238078/Images/ab238078-336565-anti-calnexin-antibody-canx-1543-protein-array.jpg)
Protein Array - Anti-Calnexin antibody [CANX/1543] (ab238078)ab238078 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)](https://www.abcam.cn/ps/products/238/ab238078/Images/ab238078-315572-anti-calnexin-antibody-canx-1543-western-blot.jpg)
Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)All lanes : Anti-Calnexin antibody [CANX/1543] (ab238078) at 2 µg/ml
Lane 1 : PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 68 kDa -
![Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)](https://www.abcam.cn/ps/products/238/ab238078/Images/ab238078-315571-anti-calnexin-antibody-canx-1543-western-blot.jpg)
Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078)Anti-Calnexin antibody [CANX/1543] (ab238078) at 2 µg/ml + Human kidney lysate
Predicted band size: 68 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab238078 被引用在 2 文献中.
- Slyusarenko M et al. Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation. Polymers (Basel) 13:N/A (2021). PubMed: 33572666
- Dai Y et al. A Multivariate Diagnostic Model Based on Urinary EpCAM-CD9-Positive Extracellular Vesicles for Prostate Cancer Diagnosis. Front Oncol 11:777684 (2021). PubMed: 34900726